FlyBase Allele Report: Dmel\klg[GD14314]

General Information

Symbol

Dmel\klg^GD14314

Species

D. melanogaster

Name

FlyBase ID

FBal0209792

Feature type

allele

Associated gene

Dmel\klg

Associated Insertion(s)

Carried in Construct

P{GD14314}

Also Known As

klg³⁶¹⁶², UAS-klgRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14314}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14314

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^hs.PB

(Matsuno et al., 2009)

abnormal memory | adult stage, with Scer\GAL4^elav.PU

(Matsuno et al., 2015)

abnormal memory | adult stage, with Scer\GAL4^nrv2.PU

(Matsuno et al., 2015)

abnormal memory | adult stage, with Scer\GAL4^repo.PU

(Matsuno et al., 2015)

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{Atpα-GSG3285-1}

(Matsuno et al., 2015)

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Matsuno et al., 2015)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of klg^GD14314 under the control of Scer\GAL4^elav.PU, Scer\GAL4^repo.PU or Scer\GAL4^nrv2.PU, but not under the control of Scer\GAL4^how-24B, results in significantly impaired long term memory performance 24 hours after spaced training in an aversive olfactory association paradigm, as compared to controls.

Expression of klg^GD14314 under the control of Scer\GAL4^elav.PU, Scer\GAL4^repo.PU or Scer\GAL4^nrv2.PU does not significantly affect anesthesia-resistant memory performance 24 hours after massed training in an aversive olfactory association paradigm, as compared to controls.

Expression of klg^GD14314 under the control of Scer\GAL4^{Atpα-GSG3285-1} or Scer\GAL4^{elav.Switch.PO} (in combination with feeding RU486 to restrict expression to adult stage from 3d before training until testing) results in significantly impaired long term memory performance 24 hours after spaced training in an aversive olfactory association paradigm, as compared to controls.

(Matsuno et al., 2015)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or semi-lethality.

(Schnorrer et al., 2010)

Heat-shock induced expression of Scer\GAL4^hs.PB driven klg^GD14314 before training results in a significant decrease in 1-day memory after spaced training.

(Matsuno et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14314

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Atpα-GSG3285-1}, klg^GD14314 has abnormal memory | adult stage | RU486 conditional phenotype, suppressible | RU486 conditional by Scer\GAL4^{Atpα-GSG3285-1}/repo^UAS.Tag:MYC

(Matsuno et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^{Atpα-GSG3285-1}, klg^GD14314 has abnormal memory | adult stage | RU486 conditional phenotype, non-suppressible by Scer\GAL4^{Atpα-GSG3285-1}/repo^{ΔHD.UAS.Tag:MYC}

(Matsuno et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of repo^{Scer\UAS.T:Hsap-MYC}, but not repo^{ΔHD.Scer\UAS.T:Hsap\MYC}, suppresses the long term memory defect seen in flies expressing klg^GD14314 under the control of Scer\GAL4^{Atpα-GSG3285-1}.

(Matsuno et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14314

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

klg³⁶¹⁶²

(Matsuno et al., 2015)

klg^GD14314

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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