UASt regulatory sequences drive expression of an inverted repeat.
Expressing rawGD7727 under the control of Scer\GAL4Toll-6-D42 (together with Dicer-2 for efficient RNAi) provides protection against (injury-induced) Wallerian degeneration of third instar larval motor neurons.
DdaE sensory neurons expressing rawGD7727 under the control of Scer\GAL4221 (together with Dicer-2 for efficient RNAi) display significantly fewer growing microtubules (EB1 comets) than controls; unlike controls, these neurons regenerate dendrites following laser dendrotomy.
Adults expressing rawGD7727 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in flightless flies.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 80-90% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.
Scer\GAL4tey-5053A, rawGD7727 has abnormal neuroanatomy | cell autonomous | third instar larval stage | conditional phenotype, non-suppressible by wndGL00282/Scer\GAL4tey-5053A
Scer\GAL4da.PU/rawGD7727, Scer\GAL80ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4da.PU, Scer\GAL80ts.αTub84B, fzrRNAi.UAS.WIZ
Scer\GAL4da.PU/rawGD7727, Scer\GAL80ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4da.PU, Scer\GAL80ts.αTub84B, fzrRNAi.UAS.WIZ
Scer\GAL4tey-5053A, rawGD7727 has axon | cell autonomous | third instar larval stage | conditional phenotype, non-suppressible by wndGL00282/Scer\GAL4tey-5053A
Scer\GAL4tey-5053A, rawGD7727 has embryonic/larval motor neuron | cell autonomous | third instar larval stage | conditional phenotype, non-suppressible by wndGL00282/Scer\GAL4tey-5053A