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General Information
Symbol
Dmel\swsGD3277
Species
D. melanogaster
Name
FlyBase ID
FBal0210718
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

1 day old Scer\GAL4loco.PU>swsGD3277 flies do not show defects in the lamina cortex compared to controls, but at 14 days old have vacuoles in the lamina cortex; at 30 days old the entire lamina cortex is filled with vacuoles and additional lesions form near the first optic chiasm (age-dependent degeneration). 14 day old Scer\GAL4repo.PU>swsGD3277 flies have vacuoles in the lamina cortex.

Small membranous glial structures can be seen in lamina cortex of 2 day old Scer\GAL4loco.PU>swsGD3277 flies, becoming larger, multilayered and more prominent in 14 day old flies; the same glial whorls can be detected in 14 day old Scer\GAL4repo.PU>swsGD3277 flies.

Scer\GAL4repo.PU>swsGD3277 flies have significantly fewer glial cells in the optic lobe compared to controls at two days old, and even more significantly at 14 days old; epithelial glia in the lamina neuropil are also significantly decreased compared to age-matched controls (2 and 14 days old) but there is no significant difference in glial cell loss between 2 and 14 day old knockdown flies. At least some of glial cells show signs of apoptosis (at 7 days old).

Expression of swsGD3277 driven by Scer\GAL4alrm.PD (targeting astrocyte-like neuropil glia) or Scer\GAL4Akap200-NP2222 (targeting cortex glia associated with neuronal cell bodies) does not cause significant defects in brains of 14 day old flies. swsGD3277 knockdown using Scer\GAL4Mz709 (targeting ensheathing and subperineurial glia) or even more so Scer\GAL4Gli-rL82.29 (targeting subperineurial glia) results in vacuolization of the lamina cortex of 14 day old flies. Small membranous glial structures are seen in the lamina cortex of Scer\GAL4Gli-rL82.29>swsGD3277 (and less so, Scer\GAL4Mz709>swsGD3277) flies. Overall, GAL4 knockdown patterns suggest phenotypes are likely due to loss of sws in the subperineurial pseudocartridge glial cells.

Expression of swsGD3277 driven by Scer\GAL4Mz709 leads to glia with stunted processes, leading to loss of glial ensheathment of neuronal cell bodies and neurites in the medulla and lobula of 14 day old adult brains, along with gaps between neurites. 1 day old Scer\GAL4repo.PU>swsGD3277 flies also show stunted glial processes and gaps between neurites (becoming more prominent in 14 day old flies, with almost all neurites surrounded by empty space). Scer\GAL4alrm.PD>swsGD3277 flies (14 day old) do not show these phenotypes. Scer\GAL4Appl.G1a>swsGD3277 flies (1 or 14 day old) do not show glial hyper-wrapping phenotypes.

Expression of swsGD3277 driven by Scer\GAL4repo.PU or Scer\GAL4loco.PU significantly reduces performance in a fast phototaxis assay (measuring percentage transitions towards light) in 2 day old flies compared to controls; this phenotype is progressive, with performance in 14 day old flies worse than in 2 day old flies. Performance is also significantly reduced when expression of swsGD3277 is driven by Scer\GAL4Gli-rL82.29, Scer\GAL4Mz709 or Scer\GAL4alrm.PD in 14 day old flies.

Adults expressing swsGD3277 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of Mmus\NTEScer\UAS.cMa significantly suppresses vacuolization of the lamina cortex in 14 day old flies with swsGD3277 driven by Scer\GAL4loco.PU.

Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments

Co-expression of swsScer\UAS.cMa significantly rescues vacuolization of the lamina cortex and partially rescues phototaxis defects in 14 day old flies with swsGD3277 driven by Scer\GAL4loco.PU. Co-expression of swsR133A.Scer\UAS (but not swsS985D.Scer\UAS) significantly (partially, not to the same extent as swsScer\UAS.cMa) reduces vacuole formation in lamina cortex and partially rescues phototaxis defects of 14 day old Scer\GAL4loco.PU>swsGD3277 flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (6)