FlyBase Allele Report: Hvir\HR13[UAS.cKa]

General Information

Symbol

Hvir\HR13^UAS.cKa

Species

H. virescens

Name

Saccharomyces cerevisiae UAS construct of Kurtovic

FlyBase ID

FBal0212280

Feature type

allele

Associated gene

Hvir\HR13

Associated Insertion(s)

Carried in Construct

P{UAS-HR13.K}

Also Known As

UAS-HR13

Transgenic product class

cDNA

(Kurtovic et al., 2007)

wild_type

(Kurtovic et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kurtovic et al., 2007)

wild_type

(Kurtovic et al., 2007)

Mutagen

in vitro construct

(Kurtovic et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-HR13.K}

Cytology

Description

UAS regulatory sequences drive expression of a Hvir\HR13 cDNA.

(Kurtovic et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hvir\HR13

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^Or67d.PF

(Benton et al., 2007)

Phenotype Manifest In

olfactory receptor neuron, with Scer\GAL4^Or67d.PF

(Benton et al., 2007)

Detailed Description

Statement

Reference

Expression of Hvir\HR13^Scer\UAS.cKa under the control of Scer\GAL4^Or67d.PF in olfactory sensory neurons that normally express Or67b confers a response to the H.virescens (Z)-11-hexadecenal pheromone in these neurons.

(Benton et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1 has abnormal smell perception | adult stage phenotype, suppressible by Snmp1¹/Snmp1²

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1 has abnormal neurophysiology | adult stage phenotype, suppressible by Snmp1¹/Snmp1²

(Gomez-Diaz et al., 2016)

Other

Statement

Reference

Hvir\HR13^UAS.cKa, Mmus\Cd36^WT.UAS, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1² has abnormal smell perception | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Mmus\Cd36^WT.UAS, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1² has abnormal neurophysiology | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^V353Y.UAS has abnormal neurophysiology | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^{T274Y.L439Y.UAS} has abnormal smell perception | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^{T274Y.L439Y.UAS} has abnormal neurophysiology | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^WT.UAS has abnormal smell perception | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^WT.UAS has abnormal neurophysiology | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^A401Y.UAS has abnormal smell perception | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^A401Y.UAS has abnormal neurophysiology | adult stage phenotype

(Gomez-Diaz et al., 2016)

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1, Snmp1¹/Snmp1², Snmp1^V353Y.UAS has abnormal smell perception | adult stage phenotype

(Gomez-Diaz et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hvir\HR13^UAS.cKa, Scer\GAL4^Or67d-GAL4-1 has adult olfactory receptor neuron Or67d phenotype, suppressible by Snmp1¹/Snmp1²

(Gomez-Diaz et al., 2016)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Adult flies expressing Hvir\HR13^Scer\UAS.cKa under the control of two copies of Scer\GAL4^Or67d-GAL4-1 cannot detect the moth Heliothis virescens sex pheromone (Z)-11-hexadecenal if they are also transheterozygous for Snmp1¹/Snmp1².

The failure of adult flies transheterozygous for Snmp1¹/Snmp1² to detect the moth Heliothis virescens sex pheromone (Z)-11-hexadecenal when they are also ectopically expressing Hvir\HR13^Scer\UAS.cKa under the control of two copies of Scer\GAL4^Or67d-GAL4-1 can be partially rescued by co-expression of Mmus\Cd36^WT.Scer\UAS : the Or67d neurons now display significant activity (measured by extracellular recordings in individual sensilla) upon (Z)-11-hexadecenal presentation.

The failure of adult flies transheterozygous for Snmp1¹/Snmp1² to detect the moth Heliothis virescens sex pheromone (Z)-11-hexadecenal when they are also ectopically expressing Hvir\HR13^Scer\UAS.cKa under the control of two copies of Scer\GAL4^Or67d-GAL4-1 can be restored by co-expression of Snmp1^WT.Scer\UAS or Snmp1^{A401Y.Scer\UAS} or Snmp1^{V353Y.Scer\UAS} or (to a lesser extent) Snmp1^{T274Y.L439Y.Scer\UAS} : The Or67d neurons display significant activity (measured by extracellular recordings in individual sensilla) upon (Z)-11-hexadecenal presentation when co-expressing any of the aforementioned Snmp1 transgenes.

(Gomez-Diaz et al., 2016)

The response to the H.virescens (Z)-11-hexadecenal pheromone that is seen in olfactory sensory neurons expressing Hvir\HR13^Scer\UAS.cKa under the control of Scer\GAL4^Or67d.PF in a wild-type background is almost completely abolished by the addition of Snmp¹/Snmp².

(Benton et al., 2007)

Flies in which the endogenous Or67d receptors in the olfactory receptor neurons have been replaced by the H.virescens Hvir\HR13 receptor (by using flies expressing Hvir\HR13^Scer\UAS.cKa under the control of Scer\GAL4^Or67d-GAL4-1 in a Or67d^GAL4-1 background) show responses to the H.virescens (Z)-11-hexadecenal pheromone that are similar to the responses shown by wild-type flies in response to the D.melanogaster cVA ([Z]-11-octadecenyl acetate) pheromone (which normally activates Or67d); in these mutant flies the T1 trichoid sensilla show a rapid firing response to (Z)-11-hexadecenal and males show suppression of courtship of virgin wild-type females that have been treated with (Z)-11-hexadecenal.

(Kurtovic et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hvir\HR13^Scer\UAS.cKa

Hvir\HR13^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Kurtovic

Secondary FlyBase IDs

References (4)

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