FlyBase Allele Report: Dmel\Hsc70-4[e3]

General Information

Symbol

Dmel\Hsc70-4^e3

Species

D. melanogaster

Name

FlyBase ID

FBal0217429

Feature type

allele

Associated gene

Dmel\Hsc70-4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hennig et al., 2006)

Progenitor genotype

Cytology

Description

A single amino acid change within the ATPase domain of Hsc70-4.

(Hennig et al., 2006)

Amino acid replacement: S40F.

(Hennig et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:15,244,478..15,244,478 [+]

Nucleotide change:

C15244478T

Amino acid change:

Reported amino acid change:

S40F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hennig et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | somatic clone

(Hennig et al., 2006)

increased cell size | somatic clone

(Hennig et al., 2006)

Phenotype Manifest In

imaginal disc

(Hennig et al., 2006)

Detailed Description

Statement

Reference

Heterozygous Hsc70-4^e3 mutants significantly alleviate the rapamycin-induced growth delay found in wild-type.

Flow cytometric analysis of mosaic wing imaginal discs reveals an increased cell size and increased G2 population in Hsc70-4^e3 mutant cells, relative to wild-type. This effects are acutely dosage-sensitive, as Hsc70-4^e3/+ heterozygous cells are also larger than wild-type.

(Hennig et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsc70-4^e3 has increased cell size | somatic clone phenotype, enhanceable by Tsc1²⁹

(Hennig et al., 2006)

Enhancer of

Statement

Reference

Hsc70-4^e3 is an enhancer of increased cell size | somatic clone phenotype of Tsc1²⁹

(Hennig et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsc70-4^e3/Hsc70-4[+], mTor^ey.Tag:FLAG has eye phenotype, suppressible by Hsc70-4^+t14

(Hennig et al., 2006)

Enhancer of

Statement

Reference

Hsc70-4^e3/Hsc70-4[+] is an enhancer of eye phenotype of mTor^ey.Tag:FLAG

(Hennig et al., 2006)

Other

Statement

Reference

Hsc70-4^e3, Tsc1²⁹ has retina phenotype

(Hennig et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygous Hsc70-4^e3 mutants cause a further reduction in eye size of Tor^{ey.hs.T:Zzzz\FLAG} mutants.

Imaginal disc cells double mutant for Hsc70-4^e3 and Tsc1²⁹ exhibit a synergistic increase in cell size as compared with their single mutant counterparts. There is an exacerbation of Tsc1²⁹-induced tissue overgrowth in the presence of the Hsc70-4^e3 mutation, in some cases resulting in a marked outgrowth of tissue in the anterior portion of the retina.

(Hennig et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Hsc70-4^e3 is rescued by Hsc70-4^+t14

(Hennig et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Hsc70-4^e3

(Hennig et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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