FlyBase Allele Report: Dmel\Stat92E[RNAi.UAS]

General Information

Symbol

Dmel\Stat92E^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0220151

Feature type

allele

Associated gene

Dmel\Stat92E

Associated Insertion(s)

Carried in Construct

P{UAS-Stat92E.RNAi}

Also Known As

UAS-STAT92E RNAi, UAS-STAT92E-IR

Key Links

Transgenic product class

RNAi_reagent

(Kim et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kim et al., 2007)

Mutagen

in vitro construct

(Kim et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Stat92E.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Stat92E sequences, which correspond to nucleotides 287-1418 (this fragment includes an intron) and nucleotides 986-487 in the opposite direction.

(Kim et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Stat92E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates rhabdoid cancer

modeled by Snr1^HMS00363, BacA\p35^UAS.cHa

(Xie et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

In a tuberculosis model by abdominal injection with Mycobacterium marinum, adult flies expressing Stat92E^{dsRNA.Scer\UAS} under the control of: Scer\GAL4^crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, present a significant decrease in the bacterial burden, and a significant increase in the survival, as compared to wild-type control flies; Scer\GAL4^Hml.Δ, in combination with gal80[ts] to restrict expression to adulthood, present a significant increase in the hemocyte numbers at day 6, but not day 5, after infection, as compared to wild-type control flies; Scer\GAL4^c564 present similar survival, as compared to wild-type control flies.

(Péan et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^crq.Unk

(Péan et al., 2017)

abnormal immune response, with Scer\GAL4^Hml.Δ

(Péan et al., 2017)

increased cell number | adult stage | conditional, with Scer\GAL4^Hml.Δ

(Péan et al., 2017)

Phenotype Manifest In

hemocyte | adult stage, with Scer\GAL4^Hml.Δ

(Péan et al., 2017)

Detailed Description

Statement

Reference

The expression of Stat92E^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 has no significant effect on the survival of adults, upon infection or not with Mycobacterium marinum, as compared to wild-type controls.

The expression of Stat92E^{dsRNA.Scer\UAS} under the control of Scer\GAL4^crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant decrease in the bacterial burden, and a significant increase in the survival of adults infected with Mycobacterium marinum, as compared to wild-type controls, whereas non-infected individuals do not present significant differences in survival as compared to wild-type controls; expression under the control of Scer\GAL4^Hml.Δ, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant increase in the hemocyte numbers at day 6, but not at day 5, after infection with Mycobacterium marinum, as compared to wild-type controls, whereas non-infected individuals do not present significant differences in hemocyte numbers as compared to wild-type controls.

(Péan et al., 2017)

Flies expressing Stat92E^{dsRNA.Scer\UAS} show a higher bacterial clearance activity than do control flies after injection with E.coli.

Flies expressing Stat92E^{dsRNA.Scer\UAS} (either using heat shock to drive expression from the minimal heat shock promoter in the P{UAS-Stat92E.RNAi} construct, or using a Scer\GAL4^da.G32 driver to drive expression) show a lower survival rate after infection with E.coli than do control flies.

(Kim et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Stat92E^RNAi.UAS/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Scer\GAL4^NP6267/Stat92E^RNAi.UAS is a suppressor of increased occurrence of cell division | adult stage phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

Stat92E^RNAi.UAS, Scer\GAL4^Act.PU is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Stat92E^RNAi.UAS is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Phenotype Manifest In

Suppressor of

Statement

Reference

Stat92E^RNAi.UAS/Scer\GAL4^NP5130 is a suppressor of adult midgut epithelium phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Stat92E^RNAi.UAS/Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Scer\GAL4^NP6267/Stat92E^RNAi.UAS is a suppressor of adult posterior midgut epithelium phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

Stat92E^RNAi.UAS, Scer\GAL4^Act.PU is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Stat92E^RNAi.UAS is a non-suppressor of adult midgut epithelium phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Scer\GAL4^NP0001/Stat92E^RNAi.UAS is a non-suppressor of intestinal stem cell | adult stage phenotype of Rnf146¹⁷

(Gultekin and Steller, 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1^KO;Cherry/Sulf1^ΔP1 mutants can be suppressed by expression of Stat92E^{dsRNA.Scer\UAS} under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).

(Takemura and Nakato, 2017)

The lower survival rate seen in flies expressing Stat92E^{dsRNA.Scer\UAS} (either using heat shock to drive expression from the minimal heat shock promoter in the P{UAS-Stat92E.RNAi} construct, or using a Scer\GAL4^da.G32 driver to drive expression) after infection with E.coli is suppressed if the flies are also heterozygous for Rel^E20.

(Kim et al., 2007)

Xenogenetic Interactions

Statement

Reference

The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^Act.PU is significantly suppressed by co-expression of Stat92E^{dsRNA.Scer\UAS}.

(Xie et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26899

P{UAS-Stat92E.RNAi}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Stat92E^RNAi.UAS

Stat92E^{dsRNA.Scer\UAS}

Stat92E^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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