FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rnf14617
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General Information
Symbol
Dmel\Rnf14617
Species
D. melanogaster
Name
FlyBase ID
FBal0348081
Feature type
allele
Associated gene
Dmel\Rnf146
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Gultekin and Steller, 2019)
Mutagen
CRISPR/Cas9
(Gultekin and Steller, 2019)
Progenitor genotype
Cytology
Description

Deletion of 4 nucleotides in the first exon of Rnf146 that introduces an early stop codon and leads to truncation of the protein.

(Gultekin and Steller, 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:19,642,642..19,642,645 [-]
Comment:

Deletion of 4 nucleotides in the first exon of Rnf146 that causes a frameshift and leads to early translation termination.

(Gultekin and Steller, 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Rnf14617/Rnf14678 transheterozygous adults do not show significant differences in the numbers of hatched eggs, pupated larvae and enclosed adult, and adults do not show overt morphological defects or changes in food uptake. Adults do show a significant increase in the numbers of intestinal stem cells/enteroblasts under either regular diet or nutrient deprivation (5% sucrose diet), as compared to heterozygous or wild-type controls.

      Rnf14617 homozygous adults show a severe decrease in lifespan under nutrient deprivation (5% sucrose diet), as compared to controls. Their midguts show an increase in cell density, with a significant increase in the numbers of intestinal stem cells/enteroblasts, but no changes in the density of enterocytes or of enteroendocrine cells and enterocytes combined, as compared to heterozygous controls.

      The adult midguts of Rnf14617/Rnf14678 transheterozygotes and of Rnf14617 homozygotes show an increase in intestinal stem cell proliferation (as shown by increased EdU and phospho-H3 staining), as compared to wild-type controls.

      Rnf14617 clonal cells in the adult midgut are able to differentiate into enterocytes and enteroendocrine cells.

      (Gultekin and Steller, 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of TnksUAS.Tag:FLAG under the control of Scer\GAL4GMR.PU in both Rnf14617/Rnf14678 transheterozygous and heterozygous backgrounds results in a rough eye phenotype, which is more severe in the homozygous backgrounds. The expression of AxnUAS.GFP in the same backgrounds does not affect eye morphology.

      (Gultekin and Steller, 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Rnf14617 is partially rescued by Rnf146UAS.Tag:MYC/Scer\GAL4NP0001

      (Gultekin and Steller, 2019)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Iduna17
      (Gultekin and Steller, 2019)
      Rnf14617
      Name Synonyms
      Secondary FlyBase IDs
        References (1)