FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\aurAKK100143
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General Information
Symbol
Dmel\aurAKK100143
Species
D. melanogaster
Name
FlyBase ID
FBal0230597
Feature type
allele
Associated gene
Dmel\aurA
Associated Insertion(s)
Carried in Construct
P{KK100143}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK100143}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK100143
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal DNA repair | third instar larval stage | conditional, with Scer\GAL4Act.PU

      (Merigliano et al., 2019)

      abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4Act.PU

      (Merigliano et al., 2019)

      abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4repo.PU

      (Shohayeb et al., 2020)

      abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Lim et al., 2017)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)

      abnormal size | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Lim et al., 2017)

      abnormal size | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)

      decreased cell number | third instar larval stage, with Scer\GAL4repo.PU

      (Shohayeb et al., 2020)

      decreased cell number | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)

      decreased size | third instar larval stage, with Scer\GAL4repo.PU

      (Shohayeb et al., 2020)

      increased cell death | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)

      increased cell number | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Lim et al., 2017)

      increased occurrence of cell division | third instar larval stage, with Scer\GAL4insc-Mz1407

      (Lim et al., 2017)

      increased occurrence of cell division | third instar larval stage, with Scer\GAL4repo.PU

      (Shohayeb et al., 2020)

      increased occurrence of cell division | third instar larval stage, with Scer\GAL4repo

      (Lim et al., 2017)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of aurAKK100143 under the control of Scer\GAL4repo.PU leads to a decrease in the brain volume, but an increase in the percentage of glial cells and neuroblasts in mitosis. It also leads to a decrease in the proportion of G1 phase glia, but an increase in the number of glial cells in S phase and in G2/M in third instar larvae.

      (Shohayeb et al., 2020)

      Expressing aurAKK100143 under the control of Scer\GAL4Act.PU results in third instar larval brain neuroblasts showing some chromosomal aberrations which are almost absent in controls. Upon X-ray irradiation, these larvae show slower repair of DNA damage (i.e. slower dissolution of γ-H2Av foci) than irradiated controls.

      (Merigliano et al., 2019)

      Expression of aurAKK100143 under the control of Scer\GAL4insc-Mz1407 leads to a significant increase in brain volume, a significant increase in the number of neuroblasts per brain lobe, and a significant increase in the proportion of neuroblasts in mitosis, as well as a significant decrease in the number of neuroblasts in S phase, and a significant increase in the number of neuroblasts in G2/M phase, but no significant difference in the number of neuroblasts in G1 phase, as compared to controls.

      Expression of aurAKK100143 under the control of Scer\GAL4repo leads to a significant decrease in third instar larval brain volume, a significant decrease in both glial and neuroblast numbers, a significant increase in the proportion of mitotic glia and neuroblasts, and a significant increase in the proportion of apoptotic neuroblasts; as well as a significant increase in third instar glial cell apoptosis, a significant increase in the proportion of glia in G2/M or S phase and a significant decrease in the proportion of glia in G1 phase, as compared to controls.

      Expression of aurAKK100143 under the control of Scer\GAL4GMR54H02 in third instar larvae does not significantly alter brain lobe volume, glial or neuroblast numbers, proportion of mitotic glia, or proportion of apoptotic glia, as compared to controls.

      (Lim et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of aurAKK100143 and Wdr62NIG.7337R under the control of Scer\GAL4repo in third instar larvae leads to significant enhancement of the decreased brain volume, the decreased glial and neuroblast numbers, and of the increased mitotic index of glia and neuroblasts, as compared to expression of either aurAKK100143 or Wdr62NIG.7337R alone.

      Co-expression of Wdr62NIG.7337R suppresses the increase in brain volume and the increased neuroblast number, but fails to suppress the increased mitotic index of neuroblasts, seen in flies expressing aurAKK100143 under the control of Scer\GAL4insc-Mz1407.

      Co-expression of aurAKK100143 enhances the increased apoptosis of glial cells in third instar larvae expressing Wdr62NIG.7337R under the control of Scer\GAL4repo.

      Co-expression of aurAKK100143 and Wdr62NIG.7337R under the control of Scer\GAL4GMR54H02 in third instar larvae leads to a significant decrease in the number of glia, a significant increase in the proportion of apoptotic glia, and a significant decrease in the number of neuroblasts, but no significant difference in brain volume or proportion of mitotic glia, as compared to controls.

      (Lim et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      aurKK100143
      aurAKK100143
      Name Synonyms
      Secondary FlyBase IDs
        References (6)