FB2026_02 , released June 18, 2026
Allele: Dmel\auxKK100927
Open Close
General Information
Symbol
Dmel\auxKK100927
Species
D. melanogaster
Name
FlyBase ID
FBal0230996
Feature type
allele
Associated gene
Dmel\aux
Associated Insertion(s)
Carried in Construct
P{KK100927}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK100927}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK100927
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease
      CEA
      (Song et al., 2017)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Parkinson's disease
      modeled by Hsap\SNCAA30P.UAS.cUa
      (Song et al., 2017)
      modeled by Hsap\SNCAUAS.cUa
      (Song et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of auxKK100927 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4ple.PU or Scer\GAL4Ddc.PU leads to age-progressive decline of the adult locomotor (climbing) abilities. Scer\GAL4ple.PU-driven expression also results in age-dependent loss of dopaminergic neurons from the PPM1/2 in the brain of aged (33 days old) but not young (3 days old) adult flies. Expression driven by the Scer\GAL4GMR58E02 driver has no evident effect on the number of dopaminergic neurons from the PAM cluster regardless of age.

      (Song et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The loss of dopaminergic PPM1/2 neurons observed even in very young adult flies expressing Hsap\SNCAA30P.Scer\UAS.cUa under the control of Scer\GAL4Ddc.PU is exacerbated further by co-expression of auxKK100927 RNAi and the number of neurons is further decreased. Simultaneous expression of the two transgenes driven by Scer\GAL4ple.PU also leads to early-onset loss of PPM1/2, which is otherwise not observed in young (3 or 10 days old) flies expressing either of the transgenes alone with this driver.

      (Song et al., 2017)
      Complementation and Rescue Data
      Rescued by

      auxKK100927 is rescued by Scer\GAL4ple.PU/auxFL.UAS.GFP

      (Song et al., 2017)

      auxKK100927 is rescued by Scer\GAL4Ddc.PU/auxFL.UAS.GFP

      (Song et al., 2017)
      Comments

      The age-progressive rapid deterioration in adult locomotion (climbing ability) characteristic for flies expressing auxKK100927 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4ple.PU or Scer\GAL4Ddc.PU can be rescued by co-expression of auxFL.Scer\UAS.T:Avic\GFP.

      Co-expression of Hsap\SNCAScer\UAS.cUa (but not Hsap\SNCAA53T.Scer\UAS.cUa) with auxKK100927 leads to significant decrease in the number of dopaminergic PPM1/2 neurons in the brains of young (3 and/or 10 days old) adult flies, which is not observed at this age when any of the transgenes is expressed alone.

      (Song et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      auxKK100927
      auxR103426
      (Song et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)