FlyBase Allele Report: Dmel\E(Pc)[JF03101]

General Information

Symbol

Dmel\E(Pc)^JF03101

Species

D. melanogaster

Name

FlyBase ID

FBal0239601

Feature type

allele

Associated gene

Dmel\E(Pc)

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03101}

Also Known As

E(Pc) RNAi, JF03101

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03101}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03101

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates hematologic cancer

modeled by hop^Tum

(Bailetti et al., 2019)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

increased cell number | spermatogenesis, with E(Pc)¹, Scer\GAL4^NP1624

(Feng et al., 2017)

increased cell number | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

lethal, with Scer\GAL4^Act5C.PI

(Dietz et al., 2015)

neoplasia | spermatogenesis, with E(Pc)¹, Scer\GAL4^NP1624

(Feng et al., 2017)

neoplasia | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

some die during pupal stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

cyst progenitor cell | increased number, with E(Pc)¹, Scer\GAL4^NP1624

(Feng et al., 2017)

cyst progenitor cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

male germline cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

male germline stem cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

posterior crossvein, with Scer\GAL4^en.PU

(Dietz et al., 2015)

spermatogonial cyst, with Scer\GAL4^NP1624

(Feng et al., 2017)

testis, with Scer\GAL4^NP1624

(Feng et al., 2017)

wing blade, with Scer\GAL4^en.PU

(Dietz et al., 2015)

wing blade, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

wing vein, with Scer\GAL4^en.PU

(Dietz et al., 2015)

Detailed Description

Statement

Reference

The expression of E(Pc)^JF03101 under the control of Scer\GAL4^NP1624 leads to increased numbers of cyst stem cells in testes (Yan- or Zfh-1-positive cells), associated with cyst stem cell differentiation defects, with a high proportion of cells abnormally co-expressing Zfh-1 (marker of early stage cyst cells) and Eya (marker of later stage cyst cells).

Unlike control testes, the expression of E(Pc)^JF03101 under the control of Scer\GAL4^NP1624 leads to frequent expansion of early stage germ cells from the apical tip region of the testes; in some there are structures resembling stem cell- or GB-like tumors with round spectrosome structures, while some other resemble spermatogonial tumors with more than 16 cells; cells within one cyst show both dotted spectrosome and branched fusome structures, as they undergo asynchronous cell cycle; some cells also express early cyst cell markers (Yan and Zfh-1). The excess early germ cell phenotype of Scer\GAL4^NP1624>E(Pc)^JF03101 is significant;y enhanced by E(Pc)¹ heterozygosity.

The expression of E(Pc)^JF03101 under the control of Scer\GAL4^upd3.PA does not lead to any detectable cellular defect in the testes.

(Feng et al., 2017)

Expression of E(Pc)^JF03101 leads to: increased pupal lethality with the adult survivors displaying small and rough eye phenotype (with the Scer\GAL4^ey.PH driver), rough adult eyes (with Scer\GAL4^GMR.PF), deformed or slightly deformed/curved (at 18[o]C) wings (with Scer\GAL4^nub.PK), increased pupal lethality with adult survivors showing held-out wings with blisters or vein defects (at 18[o]C) - incomplete posterior crossvein, ectopic vein material (with Scer\GAL4^en.PU).

(Dietz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E(Pc)^JF03101, Scer\GAL4^NP1624 has neoplasia | spermatogenesis phenotype, enhanceable by Egfr[+]/Egfr^f2

(Feng et al., 2017)

Suppressed by

Statement

Reference

E(Pc)^JF03101, Scer\GAL4^NP1624 has neoplasia | spermatogenesis phenotype, suppressible | partially by aop[+]/aop¹

(Feng et al., 2017)

E(Pc)^JF03101, Scer\GAL4^ey.PH has visible | adult stage phenotype, suppressible by htt^GD14943, Scer\GAL4^ey.PH

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by htt^GD14943, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^ey.PH has visible | adult stage phenotype, suppressible by htt^int

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by htt^int

(Dietz et al., 2015)

Other

Statement

Reference

E(Pc)^JF03101, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(Pc)^JF03101, Scer\GAL4^ey.PH has eye phenotype, suppressible by htt^GD14943, Scer\GAL4^ey.PH

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^ey.PH has eye phenotype, suppressible by htt^int

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^GMR.PF has eye phenotype, suppressible by htt^GD14943, Scer\GAL4^GMR.PF

(Dietz et al., 2015)

E(Pc)^JF03101, Scer\GAL4^GMR.PF has eye phenotype, suppressible by htt^int

(Dietz et al., 2015)

Other

Statement

Reference

E(Pc)^JF03101, Scer\GAL4^fkh.PH, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The adult eye phenotype induced by expression of E(Pc)^JF03101 under the control of either Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF is suppressed by expression of htt^GD14943 or by combination with htt^int.

(Dietz et al., 2015)

Co-expression of E(Pc)^JF03101 and p53^{Scer\UAS.A.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH results in small salivary glands and high levels of apoptotic cell death.

(Zhang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28686

y¹ v¹; P{TRiP.JF03101}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

E(Pc)^JF03101

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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