FlyBase Allele Report: Dmel\Miro[B682]

General Information

Symbol

Dmel\Miro^B682

Species

D. melanogaster

Name

FlyBase ID

FBal0239855

Feature type

allele

Associated gene

Dmel\Miro

Associated Insertion(s)

Carried in Construct

Also Known As

dMiro^B682

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Guo et al., 2005)

Mutagen

ethyl methanesulfonate

(Guo et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: W105term.

(Guo et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,037,238..24,037,238 [-]

Nucleotide change:

G24037238A

Reported nucleotide change:

G?A

Amino acid change:

W104term | Miro-PC; W104term | Miro-PD; W104term | Miro-PE; W104term | Miro-PF

Reported amino acid change:

W105term

Comment:

Reported as a W105 to UAG nonsense mutation.

(Guo et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Vagnoni et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

bouton & neuromuscular junction | larval stage

(Guo et al., 2005)

bouton & neuromuscular junction | larval stage (with Miro^Sd32)

(Guo et al., 2005)

marginal nerve | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

microtubule & bouton

(Guo et al., 2005)

mitochondrion

(Keller et al., 2011)

mitochondrion & motor neuron | larval stage (with Miro^Sd32)

(Russo et al., 2009)

mitochondrion & neuron | larval stage

(Guo et al., 2005)

mitochondrion & neuron | larval stage (with Miro^Sd23)

(Guo et al., 2005)

mitochondrion & neuron | larval stage (with Miro^Sd26)

(Guo et al., 2005)

mitochondrion & neuron | larval stage (with Miro^Sd32)

(Guo et al., 2005)

mitochondrion & somatic muscle | larval stage

(Guo et al., 2005)

mitochondrion | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

somatic muscle cell | larval stage

(Guo et al., 2005)

Detailed Description

Statement

Reference

Miro^B682/+ significantly reduces the frequency of mitochondrial transport events in axons of the wing marginal nerve, but does not significantly change the total number of mitochondria, as compared to controls.

(Vagnoni et al., 2016)

In Miro^B682 mutants the majority of mitochondria remain stranded in the neuronal soma and soma-proximal axon, whereas the distal axon and presynaptic nerve terminal are largely devoid of mitochondria. There is no evidence of NMJ degeneration or altered neuromuscular growth.

(Keller et al., 2011)

Stimulus-dependent or resting cytosolic Ca[2+] levels are not affected by the chronic lack of mitochondria in motor neuron-terminals of Miro^B682 mutants.

(Chouhan et al., 2010)

Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes.

(Dickman and Davis, 2009)

In the motor neurons of Miro^B682/Miro^Sd32 larvae, mitochondria are largely absent from neuro-muscular junctions, and are present in reduced numbers in axons, mainly concentrated close to the ventral ganglion. The proportion of motile mitochondria is significantly reduced in these cells and, unlike in wild-type, many stationary mitochondria are not clustered. In addition, the motile mitochondria (both anterograde and retrograde) are significantly shorter than in wild-type. There is also a small but significant reduction in the proportion of mitochondria that are motile and in the rate of mitochondrial flux in the axons of in motor neurons of Miro^B682/+ larvae.

(Russo et al., 2009)

Gross eye morphology is normal but phototaxis is defective in flies whose eyes are homozygous for Miro^B682.

Homozygous Miro^B682 larvae are small and have reduced muscles. They tend to wiggle on the spot, rather than moving forwards. Anterior segments show rhythmic contractions, but posterior segments are sluggish, sometimes paralyzed.

In neurons of Miro^B682/Miro^B682, Miro^Sd23/Miro^B682, Miro^Sd26/Miro^B682 or Miro^Sd32/Miro^B682 larvae, mitochondria are retained in the cell bodies and not properly distributed to neuronal processes - most neuromuscular junctions lack mitochondria. The somatic muscles of Miro^B682/Miro^B682 larvae also show highly abnormal distribution of mitochondria. However, the mitochondria in these animals show no structural abnormalities and have normal membrane potentials. In contrast to the effect on mitochondria, vesicle transport in the neurons of these animals is only weakly abnormal.

Bouton morphology and distribution is abnormal at neuromuscular junction in Miro^B682/Miro^B682 and Miro^B682/Miro^Sd32 larvae. The arrangement of microtubules in the boutons of Miro^B682/Miro^B682 larvae is also abnormal, but the actin cytoskeleton in these boutons appears normal.

In Miro^B682/Miro^B682 larvae, resting Ca2+ concentration at neuro-muscular junctions is higher than normal.

In Miro^Sd32/Miro^B682 larvae, excitatory junction potential evoked by 10Hz stimulation of neuro-muscular junctions (NMJs) starts off normal, but, unlike wild-type, rapidly decays. This phenotype is also seen in Miro^B682/Miro^B682, Miro^B682/Df(3R)mbc-R1 or Miro^B682/Miro^Sd26 larvae, but not in Miro^B682/Miro^Sd23 larvae. Prolonged 10Hz stimulation also leads to greater increases in Ca2+ concentration at NMJs than in wild-type. Miro^B682/Miro^B682 also show a significant increase in spontaneous mini excitatory junction potentials following 10Hz stimulation of neuro-muscular junctions.

(Guo et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Miro^B682 is a suppressor of abnormal neuroanatomy phenotype of α-Spec^KK101541, Scer\GAL4^elav-C155

(Keller et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Miro^B682 has mitochondrion phenotype, non-enhanceable by Scer\GAL4^elav-C155/α-Spec^KK101541

(Keller et al., 2011)

Suppressor of

Statement

Reference

Miro^B682 is a suppressor of embryonic/larval neuromuscular junction phenotype of α-Spec^KK101541, Scer\GAL4^elav-C155

(Keller et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous Miro^B682 suppresses the neuromuscular degeneration seen when α-Spec^KK101541 is expressed under the control of Scer\GAL4^elav-C155. The decrease in the number of mitochondria seen in Miro^B682 mutants is also seen in these larvae.

(Keller et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Guo et al., 2005)

(Guo et al., 2005)

(Guo et al., 2005)

(Guo et al., 2005)

Partially rescued by

Miro^Sd32/Miro^B682 is partially rescued by Scer\GAL4^RapGAP1-OK6/Miro^UAS.Tag:MYC

(Russo et al., 2009)

Miro^B682 is partially rescued by Scer\GAL4^elav-C155/Miro^UAS.cGa

(Guo et al., 2005)

Not rescued by

Miro^B682 is not rescued by Scer\GAL4^how-24B/Miro^UAS.cGa

(Guo et al., 2005)

Comments

Miro^Scer\UAS.cGa; Scer\GAL4^elav-C155 rescues most of the phenotypes due to Miro^B682/Miro^B682 : larval lethality, larval body and muscle size, distribution of mitochondria to the distal extremities of neurons, neuro-muscular junction morphology and microtubule cytoskeleton organization. None of these phenotypes are rescued by Miro^Scer\UAS.cGa; Scer\GAL4^how-24B.

(Guo et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

52003

Miro^B682/TM6C, Tb¹ Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Miro^B682

(Ogienko et al., 2025, Vagnoni et al., 2016)

dMiro^B682

(Lee et al., 2016, Melkov et al., 2016, Russo et al., 2009)

dmiro^B682

(Babic et al., 2015, Chouhan et al., 2010)

miro^B682

(Keller et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (11)

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