FlyBase Allele Report: Dmel\Dronc[30]

General Information

Symbol

Dmel\Dronc³⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0239865

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Waldhuber et al., 2005)

Progenitor genotype

Caused by aberration

Df(3L)Dronc³⁰

Cytology

Description

Imprecise excision of P{SUPor-P}Nc^KG02994 has deleted about 2.8 kb of genomic sequence, including the most of the sequence encoding Nc and CG6685.

(Waldhuber et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Waldhuber et al., 2005)

abnormal size | larval stage

(Waldhuber et al., 2005)

decreased cell death | larval stage

(Waldhuber et al., 2005)

lethal (with Df(3L)AC1)

(Waldhuber et al., 2005)

lethal (with Dronc¹¹)

(Waldhuber et al., 2005)

lethal - all die before end of larval stage

(Waldhuber et al., 2005)

some die during larval stage

(Waldhuber et al., 2005)

Phenotype Manifest In

neuroblast & larval abdominal segment 3 | late third instar larval stage

(Waldhuber et al., 2005)

neuroblast & larval abdominal segment 4 | late third instar larval stage

(Waldhuber et al., 2005)

neuroblast & larval abdominal segment 5 | late third instar larval stage

(Waldhuber et al., 2005)

neuroblast & larval abdominal segment 6 | late third instar larval stage

(Waldhuber et al., 2005)

neuroblast & larval abdominal segment 7 | late third instar larval stage

(Waldhuber et al., 2005)

Detailed Description

Statement

Reference

Nc³⁰ homozygous embryos hatch at the normal time but the larvae develop more slowly than wild-type. As a result, average body length at 72 hours after egg laying is 23% less than the average for Nc³⁰/+ controls. These larvae fail to pupariate, but continue to grow and reach a final body mass 32% greater, on average, than control larvae just prior to pupariation. The resulting giant larvae die at 10-12 days after wild-type larvae pupariate. Unlike wild-type larvae, these larvae are not induced to pupariate early by feeding with ecdysone at the mid-third instar.

Nc³⁰ homozygous larvae fail to exhibit cell death in the larval midgut, as wild-type larvae do, at end of the third larval instar (assayed by Tunel staining) or in response to feeding with ecdysone at mid-third instar (assayed by acridine orange staining).

In Nc³⁰ homozygous larvae, most of the abdominal neuroblasts in segments A3-A7 of the CNS that normally die during the late third instar persist.

X-ray induced apoptosis in wing discs is almost eliminated in Nc³⁰ homozygous third instar larvae.

(Waldhuber et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Dronc³⁰/Nc[+] is a suppressor of eye phenotype of grim^GMR.PH

(Waldhuber et al., 2005)

Dronc³⁰/Nc[+] is a suppressor of eye phenotype of rpr^GMR.PH

(Waldhuber et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc³⁰/Dronc[+] is a non-suppressor of eye phenotype of hid^GMR.PU

(Waldhuber et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Nc³⁰/+ partially suppresses the small, rough eye phenotype caused by rpr^GMR.PH or grim^GMR.PH but not that caused by W^GMR.PG.

(Waldhuber et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Dronc³⁰ is partially rescued by Dronc^UAS.EGFP/Scer\GAL4^hs.PB

(Waldhuber et al., 2005)

Not rescued by

Dronc³⁰ is not rescued by Dronc^C318S.UAS/Scer\GAL4^hs.PB

(Waldhuber et al., 2005)

Comments

Pupariation of Nc³⁰ homozygous larvae is rescued in 85% of cases by Nc^{Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^hs.PB. It also rescues normal patterns of cell death in abdominal CNS neuroblasts during the late third instar and X-ray induced cell death in wing discs.

(Waldhuber et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DRONC³⁰

(Waldhuber et al., 2005)

Dronc³⁰

Nc³⁰

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close