FlyBase Allele Report: Dmel\AMPKα[3]

General Information

Symbol

Dmel\AMPKα³

Species

D. melanogaster

Name

FlyBase ID

FBal0241421

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

Also Known As

AMPK³

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Swick et al., 2013)

Mutagen

ethyl methanesulfonate

(Swick et al., 2013)

Progenitor genotype

Cytology

Description

16bp deletion (nucleotides 422-437 relative to the translation start site of NM_057965.4). This results in amino acid Tyr141 being converted to a stop codon.

(Brenman, 2017.9.29)

16bp deletion creating a stop codon at residue 141.

(Swick et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:1,376,838..1,376,853 [+]

Comment:

A 16 bp deletion (attgccaccggcacat) in AMPKalpha resulting in a stop codon at Y141.

(Brenman, 2017.9.29)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendritic arborizing neuron | second instar larval stage

(Swick et al., 2013)

follicle cell

(Haack et al., 2013)

larval multidendritic class IV neuron

(Johnson et al., 2010)

oenocyte

(Johnson et al., 2010)

sensory neuron

(Kazgan et al., 2010)

Detailed Description

Statement

Reference

Hemizygous AMPKα³ males do not survive to adulthood.

(Onyenwoke et al., 2015)

AMPKα³ follicle cell clones show normal polarity under both well-fed and starvation conditions. Polarity is also maintained after feeding flies drugs that reduce cellular ATP levels and activate AMPK, though many egg chambers degenerate at stage 7/8.

Cells within AMPKα¹ follicle clones are larger than adjacent wild type cells, under both fed and starved conditions.

(Haack et al., 2013)

Homozygous mutants display a completely penetrant phenotype of significantly enlarged plasma membrane domains in dendrites but not in axonal compartments of da neurons in second instar larvae.

(Swick et al., 2013)

Mutant embryos have a normal somatic muscle pattern. The number of nuclei in the segmental border muscle is normal.

(Tixier et al., 2013)

Heterozygous flies are more resistant to P. entomophila infection than control flies.

(Chakrabarti et al., 2012)

SNF1A³ mutants exhibit aberrant morphology in class IV multi-dendritic neurons.

SNF1A³ mutants die as larvae and are characteristically smaller than wild-type larvae of the same age.

The majority of SNF1A³ mutants die between the fifth and seventh day of the third instar larval stage. Feeding these larvae with Rapamycin increases the percentage of mutant larvae surviving to the seventh day.

Under fed conditions, SNF1A³ larvae show significantly more and larger lipid droplets in oenocytes compared to controls, resembling the starved phenotype of wild-type oenocytes.

(Johnson et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AMPKα³ has lethal - all die during P-stage | recessive phenotype, suppressible by Scer\GAL4^Tub.PU/S6k^KQ.UAS

(Onyenwoke et al., 2015)

AMPKα³ has lethal - all die during P-stage | recessive phenotype, suppressible by Trpml¹

(Onyenwoke et al., 2015)

AMPKα³ has lethal - all die during P-stage | recessive phenotype, suppressible by Trpml²

(Onyenwoke et al., 2015)

AMPKα³ has lethal - all die during P-stage | recessive phenotype, suppressible by Scer\GAL4^Act.PU/Trpml^JF01239

(Onyenwoke et al., 2015)

NOT suppressed by

Statement

Reference

AMPKα³ has lethal - all die during P-stage | recessive phenotype, non-suppressible by Scer\GAL4^Tub.PU/mTor^TED.UAS

(Onyenwoke et al., 2015)

AMPKα³ has lethal - all die during P-stage | recessive phenotype, non-suppressible by Trpml^UAS.cVa/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

AMPKα³ has lethal - all die during P-stage | recessive phenotype, non-suppressible by SNF4Aγ^UAS.GFP/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The pupal-stage lethality of hemizygous AMPKα³ males can be partially rescued by expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^tub.PU but not by expression of either of the following: Tor^TED.Scer\UAS, Trpml^Scer\UAS.cVa, SNF4Aγ^{Scer\UAS.T:Avic\GFP}.

The pupal-stage lethality of hemizygous AMPKα³ males can be partially rescued by combination with either Trpml¹ or Trpml².

The pupal-stage lethality of hemizygous AMPKα³ males can be partially rescued by expression of Trpml^JF01239 RNAi under the control of Scer\GAL4^Act.PU.

(Onyenwoke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

AMPKα³ is rescued by Scer\GAL4^109(2)80/AMPKα^UAS.cMa

(Swick et al., 2013)

AMPKα³ is rescued by Scer\GAL4^Ubi.PU/AMPKα^UAS.mCherry

(Swick et al., 2013)

AMPKα³ is rescued by AMPKα^UAS.cMa/Scer\GAL4^Ubi.PU

(Swick et al., 2013)

AMPKα³ is rescued by Scer\GAL4^109(2)80/AMPKα^UAS.cMa

(Kazgan et al., 2010)

Partially rescued by

AMPKα³ is partially rescued by AMPKα^UAS.cMa/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Not rescued by

AMPKα³ is not rescued by Scer\GAL4^109(2)80/AMPKα^ΔC.UAS

(Kazgan et al., 2010)

Comments

The pupal stage lethality observed in either AMPKα¹ or AMPKα³ hemizygous males can be partially rescued by expression of AMPKα^Scer\UAS.cMa under the control of Scer\GAL4^tub.PU.

(Onyenwoke et al., 2015)

Scer\GAL4^Ubi-mediated expression of SNF1A^{Scer\UAS.T:Disc\RFP-mCherry} rescues viability and fertility in SNF1A³ mutants.

Scer\GAL4^Ubi-mediated expression of SNF1A^Scer\UAS.cMa rescues viability in SNF1A³ mutants.

Scer\GAL4^109(2)80-mediated expression of SNF1A^Scer\UAS.cMa rescues the dendritic phenotype of SNF1A³ mutants.

(Swick et al., 2013)

Expression of SNF1A^Scer\UAS.cMa under the control of Scer\GAL4^109(2)80 rescues the neuronal defects of SNF1A³ mutants.

Expression of SNF1A^ΔC.Scer\UAS under the control of Scer\GAL4^109(2)80 fails to rescue the neuronal defects of SNF1A³ mutants.

(Kazgan et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33831

AMPKα³/FM7i

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

AMPK³

(Johnson et al., 2010)

AMPK^α3

(Tixier et al., 2013)

AMPKα³

(Brenman, 2017.9.29, Kazgan et al., 2010)

Ampkα³

(van den Ameele and Brand, 2019)

SNF1A³

(Onyenwoke et al., 2015, Radermacher et al., 2014)

ampk³

(Gailite et al., 2015)

ampkα³

(Haack et al., 2013, Swick et al., 2013, Chakrabarti et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close