FlyBase Allele Report: Dmel\Pc[NIG.32443R]

General Information

Symbol

Dmel\Pc^NIG.32443R

Species

D. melanogaster

Name

FlyBase ID

FBal0243842

Feature type

allele

Associated gene

Dmel\Pc

Associated Insertion(s)

Carried in Construct

P{NIG.32443R}

Also Known As

Pc RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Saj et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Saj et al., 2010)

Mutagen

in vitro construct

(Saj et al., 2010)

Progenitor genotype

Carried in construct

P{NIG.32443R}

Cytology

Description

UASt regulatory sequences drive expression of a Pc hairpin interfering RNA (IR) construct.

(Saj et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-32443R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

increased cell number | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

Phenotype Manifest In

cyst cell of testis, with Scer\GAL4^C587

(Zhang et al., 2017)

cyst progenitor cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

germinal proliferation center | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

male germline stem cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

primary gonial cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

Detailed Description

Statement

Reference

The adulthood-only expression of Pc^NIG.32443R (using Gal80[ts]) driven by Scer\GAL4^C587, but not by Scer\GAL4^VP16.nos.UTR, Scer\GAL4^A3.eya or Scer\GAL4^hh-Gal4, leads to adult testes exhibiting a significant increase in the number of cyst stem cells, a reciprocal decrease of differentiated cyst cells, an increase in the number of early stage germline cells, and a strong increase in the number of cells undergoing cell division at the apical testis tip, as compared to controls. This Scer\GAL4^C587-dependent expression also leads cell non-autonomous induction of both somatic (Zfh1) and germline (Vasa) lineage markers in some somatic-derived cells, as compared to controls.

(Zhang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pc^NIG.32443R, Scer\GAL4^C587 has increased cell number | spermatogenesis phenotype, suppressible | partially by Abd-B^GLV21012, Scer\GAL4^C587

(Zhang et al., 2017)

Pc^NIG.32443R, Scer\GAL4^C587 has increased cell number | spermatogenesis phenotype, suppressible | partially by wg^HMS00794, Scer\GAL4^C587

(Zhang et al., 2017)

NOT suppressed by

Statement

Reference

Pc^NIG.32443R, Scer\GAL4^C587 has increased cell number | spermatogenesis phenotype, non-suppressible by dpp^JF01091, Scer\GAL4^C587

(Zhang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pc^NIG.32443R, Scer\GAL4^C587 has cyst progenitor cell | spermatogenesis phenotype, suppressible | partially by Abd-B^GLV21012, Scer\GAL4^C587

(Zhang et al., 2017)

Pc^NIG.32443R, Scer\GAL4^C587 has cyst progenitor cell | spermatogenesis phenotype, suppressible | partially by wg^HMS00794, Scer\GAL4^C587

(Zhang et al., 2017)

NOT suppressed by

Statement

Reference

Pc^NIG.32443R, Scer\GAL4^C587 has cyst progenitor cell | spermatogenesis phenotype, non-suppressible by dpp^JF01091, Scer\GAL4^C587

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of cyst stem cells in adult testes resulting from the adulthood-only expression of Pc^NIG.32443R under the control of Scer\GAL4^C587 and Gal80[ts] is partially suppressed by the co-expression of Abd-B^GLV21012 or wg^HMS00794, but not of dpp^JF01091.

(Zhang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

32443R-1

P{NIG.32443R}1

32443R-4

P{NIG.32443R}4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pc^NIG.32443R

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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