FlyBase Allele Report: Dmel\Atf3[76]

General Information

Symbol

Dmel\Atf3⁷⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0245993

Feature type

allele

Associated gene

Dmel\Atf3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

P{EPgy2}Atf3^EY02562

(Sekyrova et al., 2010)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes the entire bZIP domain.

(Sekyrova et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Rynes et al., 2012)

decreased body size | male

(Rynes et al., 2012)

lethal

(Rynes et al., 2012)

lethal - all die before end of larval stage

(Sekyrova et al., 2010)

short lived | male

(Rynes et al., 2012)

some die during larval stage

(Sekyrova et al., 2010)

Phenotype Manifest In

embryonic/larval fat body

(Sekyrova et al., 2010)

lipid droplet

(Rynes et al., 2012)

Detailed Description

Statement

Reference

Homozygous larvae die throughout the larval stages under normal culture conditions. If competition with wild-type siblings is minimised, the lethal phase is postponed to the pupal stage and a small number of adults are obtained. In this case, the mutant larvae grow more slowly than controls (third instar mutant larvae are only 60% the body mass of control larvae) and their organs are undersized. Pupariation and adult eclosion is delayed by 2 to 3 days compared to wild type. Mutant adult males are small and short lived.

The population of Acetobacter sp. and Lactobacillus sp. in the gut is increased in mutant larvae compared to controls.

Fat body cells of feeding mutant third instar larvae contain lipid droplets of variable size, with enlarged droplets being the most common. Enlarged lipid droplets are also seen in the mutant midgut. The mutant larvae contain almost twice the normal amount of stored triacylglycerols, and the levels of diacylglycerols and free fatty acids are also increased compared to wild type. The amounts of circulating carbohydrate (glucose and trehalose) and stored glycogen are not significantly different from wild type. The larvae primarily metabolise carbohydrates and their total metabolism is reduced compared to wild type.

(Rynes et al., 2012)

Most mutant animals die soon after hatching and during all three larval stages. Only 2% of the mutant animals reach the third instar, but they die before metamorphosis as defective pseudopuparia. The mutant moribund larvae have abnormally enlarged lipid droplets in their fat body.

Mutant embryos develop normally, and no not show a dorsal open defect, even when derived from females containing homozygous germline clones.

(Sekyrova et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atf3⁷⁶ has lethal phenotype, suppressible | partially by Rel^E20/Rel[+]

(Rynes et al., 2012)

Atf3⁷⁶ has lethal phenotype, suppressible | partially by Scer\GAL4^fat/Hsap\ATF3^UAS.Tag:FLAG

(Rynes et al., 2012)

Atf3⁷⁶ has lethal phenotype, suppressible | partially by Scer\GAL4^C7/Hsap\ATF3^UAS.Tag:FLAG

(Rynes et al., 2012)

NOT suppressed by

Statement

Reference

Atf3⁷⁶ has lethal phenotype, non-suppressible by foxo^25J13/foxo[+]

(Rynes et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atf3⁷⁶ has lipid droplet phenotype, suppressible | partially by Scer\GAL4^fat/Hsap\ATF3^UAS.Tag:FLAG

(Rynes et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The increased levels of triacylglycerols and diacylglycerols which are seen in Atf3⁷⁶ larvae are reduced if the larvae are also heterozygous for either Rel^E20 or foxo^25J13. In addition, heterozygosity for Rel^E20 also reduces the increased levels of free fatty acids seen in Atf3⁷⁶ larvae.

Atf3⁷⁶ ; Rel^E20/+ animals show increased survival to adulthood compared to Atf3⁷⁶ animals, while heterozygosity for foxo^25J13 has no effect on Atf3⁷⁶ survival.

(Rynes et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ATF3^{Scer\UAS.T:Zzzz\FLAG} under the control of either Scer\GAL4^fat or Scer\GAL4^C7 significantly improves the survival of Atf3⁷⁶/Y males.

Expression of Hsap\ATF3^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^fat rescues the abnormally large lipid droplets and high triacylglycerol levels seen in Atf3⁷⁶ larvae.

(Rynes et al., 2012)

Complementation and Rescue Data

Rescued by

Atf3⁷⁶ is rescued by Atf3^{CH321-51N24.EGFP}

(Rynes et al., 2012)

Atf3⁷⁶ is rescued by Atf3^UAS.cSa/Scer\GAL4^arm.PS

(Sekyrova et al., 2010)

Comments

One copy of Atf3^{CH321-51N24.T:Avic\GFP-EGFP} completely rescues the developmental delay and adult eclosion of Atf3⁷⁶/Y males and permits the survival of homozygous Atf3⁷⁶ females.

Atf3^Scer\UAS.cSa can rescue the lethality of Atf3⁷⁶/Y males or can reduce survival to below that of Atf3⁷⁶/Y males when expressed under the control of either Scer\GAL4^fat or Scer\GAL4^C7, depending on the insertion line used.

Atf3^Scer\UAS.cSa in the absence of a Scer\GAL4 driver rescues the abnormally large lipid droplets and high triacylglycerol levels seen in Atf3⁷⁶ larvae.

(Rynes et al., 2012)

Some Atf3⁷⁶ hemizygotes are rescued to adulthood by expression of Atf3^Scer\UAS.cSa under the control of Scer\GAL4^arm.PS.

(Sekyrova et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atf3⁷⁶

atf3⁷⁶

(Donohoe et al., 2018, Chakrabarti et al., 2014, Rynes et al., 2012, Sekyrova et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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