FlyBase Allele Report: Dmel\E2f1[UASp.EGFP]

General Information

Symbol

Dmel\E2f1^UASp.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0248936

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-E2f1.WT}

Also Known As

UAS-GFP-E2f1

Key Links

Transgenic product class

wild_type

(Shibutani et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Shibutani et al., 2008)

Mutagen

in vitro construct

(Shibutani et al., 2008)

Progenitor genotype

Carried in construct

P{UASp-GFP-E2f1.WT}

Cytology

Description

UASp regulatory sequences drive expression of wild-type E2f1 which is tagged with EGFP.

(Shibutani et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

E2f1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}

(Kwon et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^en-e16E

(Davidson and Duronio, 2012)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2021)

increased occurrence of cell division | embryonic stage, with Scer\GAL4^arm.PS

(Shibutani et al., 2008)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^arm.PS

(Shibutani et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of E2f1^UASp.EGFP under the control of Scer\GAL4^esg.PU leads to increased mitoses in the adult gut.

(Zhang et al., 2021)

Approximately 12% of trypsin-dissociated posterior compartment cells from third instar larval wing discs expressing E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E are in S phase, compared to ~27% of controls. The rate of proliferation in these cells is increased, with ~11% of cells having a G1 DNA content compared to around 28% in controls.

Expression of E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E does not increase apoptosis levels in the posterior compartment of intact third instar larval wing disc cells compared to controls. The same is seen when the wing disc cells are trypsin-dissociated and quantified using flow cytometry.

The cells of the second mitotic wave in eye discs expressing E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU show the stereotypical pattern of S phase seen in wild type. As in wild type flies, very few cells enter apoptosis immediately posterior to the morphogenetic furrow and levels of DNA damage are similar to controls.

(Davidson and Duronio, 2012)

Expression of E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^arm.PS results in ectopic BrdU incorporation in the anterior and posterior midgut in embryos.

(Shibutani et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E2f1^UASp.EGFP, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by cbt^NIG.4427R, Scer\GAL4^esg.PU

(Zhang et al., 2021)

Suppressor of

Statement

Reference

E2f1^UASp.EGFP, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

Other

Statement

Reference

BacA\p35^UAS.cHa, E2f1^UASp.EGFP, Scer\GAL4^en-e16E has lethal phenotype

(Davidson and Duronio, 2012)

BacA\p35^UAS.cHa, E2f1^UASp.EGFP, Scer\GAL4^en-e16E has abnormal size | third instar larval stage phenotype

(Davidson and Duronio, 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

E2f1^UASp.EGFP, Scer\GAL4^esg.PU has gut epithelium | adult stage phenotype, suppressible | partially by cbt^NIG.4427R, Scer\GAL4^esg.PU

(Zhang et al., 2021)

E2f1^UASp.EGFP, Scer\GAL4^esg.PU has intestinal stem cell | adult stage phenotype, suppressible | partially by cbt^NIG.4427R, Scer\GAL4^esg.PU

(Zhang et al., 2021)

Suppressor of

Statement

Reference

E2f1^UASp.EGFP, Scer\GAL4^ppk.PU is a suppressor | partially of larval multidendritic class IV neuron | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

Other

Statement

Reference

BacA\p35^UAS.cHa, E2f1^UASp.EGFP, Scer\GAL4^en-e16E has wing disc | posterior | third instar larval stage phenotype

(Davidson and Duronio, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP} and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E produces a range of morphological defects in third instar larval wing discs. Whilst some discs appear normal, most display varying degrees of overgrowth in the posterior compartment.

(Davidson and Duronio, 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34058

w^*; P{UASp-GFP-E2f1.WT}4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2f1^{Scer\UAS.P\T.T:Avic\GFP-EGFP}

E2f1^UASp.EGFP

E2f^{Scer\UAS.P\T.T:Avic\GFP-EGFP}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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