FlyBase Allele Report: Dmel\Gug[75QN.UAS]

General Information

Symbol

Dmel\Gug^75QN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258445

Feature type

allele

Associated gene

Dmel\Gug

Associated Insertion(s)

Carried in Construct

P{UAS-Atro.75QN}

Key Links

Transgenic product class

trinucleotide_repeat_expansion

(Nisoli et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Nisoli et al., 2010)

Mutagen

in vitro construct

(Nisoli et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Atro.75QN}

Cytology

Description

UASt regulatory sequences drive expression of a modified form of Gug carrying a 75Q stretch in the middle of a polyprolin domain.

(Nisoli et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gug

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of dentatorubral-pallidoluysian atrophy

CEA

(Nisoli et al., 2010)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of dentatorubral-pallidoluysian atrophy

is exacerbated by Atg1^GS10797

(Nisoli et al., 2010)

is exacerbated by mTor^TED.UAS

(Nisoli et al., 2010)

is exacerbated by ft⁸

(Napoletano et al., 2011)

is exacerbated by ft^G-rv

(Napoletano et al., 2011)

is exacerbated by sav³

(Napoletano et al., 2011)

is ameliorated by wts^G4808

(Napoletano et al., 2011)

is ameliorated by dachs^GC13

(Napoletano et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | progressive, with Scer\GAL4^GMR.PF

(Napoletano et al., 2011)

lethal, with Scer\GAL4^Eaat1.PR

(Nisoli et al., 2010)

lethal, with Scer\GAL4^elav.PU

(Nisoli et al., 2010)

Phenotype Manifest In

autophagosome, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

autophagosome | increased number, with Scer\GAL4^ninaE.PT

(Robin et al., 2019)

eye, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

eye, with Scer\GAL4^ninaE.PT

(Nisoli et al., 2010)

eye photoreceptor cell, with Scer\GAL4^ninaE.PT

(Napoletano et al., 2011, Nisoli et al., 2010)

eye photoreceptor cell | drug conditional, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

eye photoreceptor cell | progressive, with Scer\GAL4^GMR.PF

(Napoletano et al., 2011)

retina | temperature conditional, with Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Detailed Description

Statement

Reference

The expression of Gug^75QN.UAS under the control of Scer\GAL4^ninaE.PT induces the formation of autophagosomes (Atg8a puncta) in the photoreceptor cells of the retina, as compared to controls.

(Robin et al., 2019)

Flies expressing Gug^{75QN.Scer\UAS} under the control of Scer\GAL4^GMR.PF during the adult stage (using the a temperature-sensitive Scer\GAL80 line to control the timing of expression) show weak neurodegeneration in the eye at 14 days of age.

28 day old flies expressing Gug^{75QN.Scer\UAS} under the control of Scer\GAL4^ninaE.PT show loss of photoreceptor cells in approximately 40% of ommatidia.

(Napoletano et al., 2011)

Expression of Gug^{75QN.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PU results in progressive depigmentation. At 14 days old, degenerating photoreceptor cells display large autophagosomes.

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^ninaE.PT results in degeneration, characterised by loss of photoreceptor cells.

Expression of Gug^{75QN.Scer\UAS} in the glia under the control of Scer\GAL4^Eaat1.PR results in reduced animal viability.

Expression of Gug^{75QN.Scer\UAS} in the neurons under the control of Scer\GAL4^elav.PU results in reduced animal viability.

Expression of Gug^{75QN.Scer\UAS} in the photoreceptor neurons under the control of Scer\GAL4^GMR.PU in flies fed on 1μM rapamycin for 2 or 7 days both result in a reduction in the number of photoreceptor cells per ommatidium.

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue.

(Nisoli et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, enhanceable by ft⁸/ft[+]

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, enhanceable by ft^G-rv/ft[+]

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, enhanceable by sav[+]/sav³

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^GMR.PU has autophagosome phenotype, enhanceable by mTor^TED.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^75QN.UAS, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by mTor^TED.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^75QN.UAS, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by Atg5^RNAi.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Gug^75QN.UAS, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, enhanceable by Atg1^GS10797, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

NOT Enhanced by

Statement

Reference

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, non-enhanceable by wts^x1/wts[+]

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, non-enhanceable by yki[+]/yki^B5

(Napoletano et al., 2011)

Suppressed by

Statement

Reference

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, suppressible by wts^GMR.PT

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, suppressible by dachs^GC13

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^GMR.PU has retina | temperature conditional phenotype, suppressible by DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

NOT suppressed by

Statement

Reference

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, non-suppressible by wts^x1/wts[+]

(Napoletano et al., 2011)

Gug^75QN.UAS, Scer\GAL4^ninaE.PT has eye photoreceptor cell phenotype, non-suppressible by yki[+]/yki^B5

(Napoletano et al., 2011)

NOT Suppressor of

Statement

Reference

Gug^75QN.UAS/Scer\GAL4^GMR.PU is a non-suppressor of eye | somatic clone phenotype of Atg1^Δ3D

(Nisoli et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The photoreceptor degeneration caused by Gug^{75QN.Scer\UAS} under the control of Scer\GAL4^ninaE.PT is significantly suppressed inside d^GC13 clones.

(Napoletano et al., 2011)

Co-expression of Tor^TED.Scer\UAS and Gug^{75QN.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PU results in the formation of gigantic autophagolysosomes.

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is suppressed by co-expression of DnaJ-1^Scer\UAS.cKa.

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5^{dsRNA.Scer\UAS}.

(Nisoli et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Gug^{75QN.Scer\UAS}

Gug^75QN.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

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