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General Information
Symbol
Dmel\Atg5dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0176391
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Atg5-RNAi, UAS-atg5RNAi, Atg5-RNAI, UAS-Atg5IR, Atg5 RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Inverted repeat construct, with 3'-5'.5'-3' inverted repeats whose expression is driven by UASt.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Atg5dsRNA.UAS under the control of Scer\GAL4arm.PU does not affect sensitivity to starvation.

Adults expressing Atg5dsRNA.UAS under the control of either Scer\GAL4hs.PB, Scer\GAL4elav-C155 or Scer\GAL4repo infected with ZIKV present an increased viral load in the head, as compared to infected controls.

Expressing Atg5dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 has no effect on the increase in sleep duration seen in wild type flies in response to rapamycin.

Expression of Atg5dsRNA.Scer\UAS under the control of Scer\GAL4ChAT.7.4 results in a significant decrease in life span as compared to control flies.

Flies expressing Atg5dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486 show reduced lysotracker red staining of the adult midgut compared to controls in both fed and fasting flies.

Adult expression of Atg5dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486 has no effect on adult lifespan.

The lifespan of flies injected with E.coli is significantly reduced by expression of Atg5dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR in the presence of RU486. These flies show a significant increase in bacterial load compared to control flies.

Constitutive induction of Atg5 has no sigificant effect on the size or growth rate of wild type animals. Clonal expression in the fat body causes hypersensitivity to starvation. Inhibition of Atg5 causes rapamycin sensitivity.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference

Atg5dsRNA.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug75QN.UAS, Scer\GAL4GMR.PU

Atg5dsRNA.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug66QC.UAS, Scer\GAL4GMR.PU

Atg5dsRNA.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of GugUAS.cCa, Scer\GAL4GMR.PU

Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Knockdown of Atg5, through expression of Atg5dsRNA.Scer\UAS, does not impact on the initial growth of Pten117 clones with reduced slif function (through expression of slifUY681).

The ability of AcnScer\UAS.T:Hsap\MYC expressed under the control of Scer\GAL4Lsp2.PH to induce autolysosome formation in the fat body in the absence of starvation is suppressed by co-expression of Atg5dsRNA.Scer\UAS.

Expression of GugScer\UAS.cCa in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5dsRNA.Scer\UAS.

Expression of Gug66QC.Scer\UAS in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5dsRNA.Scer\UAS.

Expression of Gug75QN.Scer\UAS in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5dsRNA.Scer\UAS.

Xenogenetic Interactions
Statement
Reference

Co-expression of Atg5dsRNA.Scer\UAS leads to partial suppression of the lifespan reduction seen in flies expressing Hsap\APPAβ42.Scer\UAS under the control of Scer\GAL4ChAT.7.4.

Flies co-expressing Atg5dsRNA.Scer\UAS and Hsap\ATXN3fl.Q84.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PU show loss of retinal integrity.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Atg5dsRNA.Scer\UAS
Atg5dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (22)