FlyBase Allele Report: Dmel\CYLD[UAS.cXa]

General Information

Symbol

Dmel\CYLD^UAS.cXa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Xue

FlyBase ID

FBal0260901

Feature type

allele

Associated gene

Dmel\CYLD

Associated Insertion(s)

Carried in Construct

P{UAS-CYLD.X}

Key Links

Transgenic product class

cDNA

(Xue et al., 2007)

wild_type

(Xue et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Xue et al., 2007)

wild_type

(Xue et al., 2007)

Mutagen

in vitro construct

(Xue et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-CYLD.X}

Cytology

Description

UASt regulatory sequences drive expression of CYLD (2.1kb XhoI-EcoRV fragment from the SD04636 cDNA clone).

(Xue et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CYLD

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Phenotype Manifest In

scutellum, with Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Detailed Description

Statement

Reference

Expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^GMR.PF does not result in a mutant phenotype in the eye.

Expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^pnr-MD237 results in a slightly reduced scutellum compared to wild type.

(Xue et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has visible phenotype, suppressible by Traf6^RNAi.UAS, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has visible phenotype, suppressible by Tak1^UAS.cMa, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

NOT suppressed by

Statement

Reference

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has visible phenotype, non-suppressible by wgn^RNAi.UAS, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has scutellum phenotype, suppressible by Traf6^RNAi.UAS, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has scutellum phenotype, suppressible by Tak1^UAS.cMa, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

NOT suppressed by

Statement

Reference

CYLD^UAS.cXa, Scer\GAL4^pnr-MD237 has scutellum phenotype, non-suppressible by wgn^RNAi.UAS, Scer\GAL4^pnr-MD237

(Xue et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Traf6^{dsRNA.Scer\UAS} or Tak1^Scer\UAS.cMa fully suppresses the reduced scutellum phenotype caused by expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^pnr-MD237.

Co-expression of wgn^{dsRNA.Scer\UAS} does not suppress the reduced scutellum phenotype caused by expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^pnr-MD237.

(Xue et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

CYLD^UAS.cXa/Scer\GAL4^Tub.PU rescues CYLD^mr4

(Xue et al., 2007)

CYLD^UAS.cXa/Scer\GAL4^GMR.PF rescues CYLD^mr4

(Xue et al., 2007)

Comments

Expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^tub.PU rescues both the shortened lifespan increased sensitivity to paraquat which is seen in CYLD^mr4 mutant adults (animals homozygous for Df(2L)CYLD^mr4 and carrying P{LX107} to rescue the function of CG13138 and nmd).

Expression of CYLD^Scer\UAS.cXa under the control of Scer\GAL4^GMR.PF suppresses the ability of CYLD^mr4 to suppress the small-eye phenotype caused by expression of egr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PF.

(Xue et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

82152

w^*; P{UAS-CYLD.X}2

82153

w^*; P{UAS-CYLD.X}3

Notes on Origin

Discoverer