FB2025_05 , released December 11, 2025
Allele: Dmel\diaΔDad.UASp.EGFP
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General Information
Symbol
Dmel\diaΔDad.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277860
Feature type
allele
Associated gene
Dmel\dia
Associated Insertion(s)
Carried in Construct
P{UASp-dia.ΔDad.EGFP}
Also Known As
UAS-ΔDAD-Dia-GFP
Key Links
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
in vitro construct
(Homem and Peifer, 2009)
Progenitor genotype
Carried in construct
P{UASp-dia.ΔDad.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of dia lacking the C-terminal dia autoinhibitory domain. The protein is tagged at the N-terminal end with EGFP (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker). Encodes a constitutively active protein.

(Homem and Peifer, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
dia
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4sns.PK results in defects in myoblast fusion. Fusion competent myoblasts show a decrease in the volume of F-actin foci and an increase in the frequency of filopodia emanating from an F-actin focus compared to controls.

      (Haralalka et al., 2014)

      Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E results in a dramatic increase in the number of filopodia in the epidermal cells. The filopodia are no longer confined to leading edge cells but cover the apical surface of all epidermal cells. In the leading edge cells, filopodia are seen all over the cell, rather than being confined to the leading edge. At the leading edge, the number of filopodia is substantially increased and broad lamellipodia terminating in many filopodia are also seen. Individual filopodia are blunter than in wild type and the lifetime of each filopodium is more than doubled.

      (Homem and Peifer, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of enaScer\UAS.T:Avic\GFP and diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos induces protrusions in epidermal cells that are different from those produced by expression of either construct alone. Co-expression results in lamellipodia both at the leading edge and in more ventral epidermal cells, at the expense of filopodia.

      (Homem and Peifer, 2009)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E does not suppress the ability of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP to induce elevated numbers of filopodia on leading edge cells or on more ventral epidermal cells. The lifetime of the filopodia is still longer than normal in the co-expressing embryos. The number of lamellipodia and lamellipodial area is significantly reduced in the co-expressing leading edge cells compared to wild type.

      (Homem and Peifer, 2009)
      Complementation and Rescue Data
      Rescues

      diaΔDad.UASp.EGFP/Scer\GAL4neur-GAL4-A101 rescues dia5

      (Peng et al., 2012)
      Comments

      Expression of diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP in the sensory organ precursor lineage (using Scer\GAL4neur-GAL4-A101) in large homozygous dia5 clones in the leg restores socket cell protrusions and the formation of associated bract cells in the clones.

      (Peng et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP
      diaΔDad.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (10)