FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\GABA-B-R2RNAi.UAS
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General Information
Symbol
Dmel\GABA-B-R2RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0278296
Feature type
allele
Associated gene
Dmel\GABA-B-R2
Associated Insertion(s)
Carried in Construct
P{UAS-GABA-B-R2.RNAi}
Also Known As
UAS-GABABR2-RNAi, UAS-GABAB-RNAi
Key Links
Transgenic product class
RNAi_reagent
(Root et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Root et al., 2008)
Mutagen
in vitro construct
(Root et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-GABA-B-R2.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of a 150bp inverted repeat of the 3' end of the GABA-B-R2 coding region with a 50bp linker sequence.

(Root et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
GABA-B-R2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Male flies expressing GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4GABA-B-R2.PR display a slight, but significantly reduced life span compared to controls.

      Male flies expressing GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4Ilp2.PW show significantly reduced survival in response to starvation compared to controls. The phenotype is slightly stronger when GABA-B-R2dsRNA.Scer\UAS is expressed under the control of Scer\GAL4Ilp3.PB. Flies also show a reduced lifespan under both drivers when exposed to dessication (no food or water).

      Male flies expressing GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4Ilp2.PW show a strongly increased drop in whole body lipid levels in the 12h after starvation compared to controls. The drop in whole body trehalose that is normally seen in the 12 hours following starvation in wild type flies is significantly diminished.

      Strongly reduced survival is seen in response to starvation and dessication when GABA-B-R2dsRNA.Scer\UAS is expressed in male flies under the control of Scer\GAL4ey-OK107. No alteration to starvation response is seen when GABA-B-R2dsRNA.Scer\UAS is expressed under the control of Scer\GAL4Mef2.247.

      Expression of GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4Ilp2.PW, Scer\GAL4Ilp3.PB or Scer\GAL4ey-OK107 does not affect growth. Late third instar larvae, pupae and 4-6 day old males and females all have similar weights to controls.

      (Enell et al., 2010)

      Expression of GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4Orco.2.642.T:Hsim\VP22 increases the response of ORNs to electrical stimulation.

      Expression of GABA-B-R2dsRNA.Scer\UAS under the control of Scer\GAL4Or47b.PC increases the gain of the post-synaptic PN firing frequency.

      Expression of GABA-B-R2dsRNA.Scer\UAS in pheromone sensing ORNs in the DA1 and VA1lm glomeruli, under the control of Scer\GAL4Orco.2.642.T:Hsim\VP22, results in a reduction in male sensitivity to female pheromones. Within 30 minutes, approximately 60% of control flies have located a female fly in controlled study, whereas only 30% of GABA-B-R2dsRNA.Scer\UAS-expressing flies are able to find the female.

      Courtship latency is unaffected by expression of GABA-B-R2dsRNA.Scer\UAS in Or47b ORNS (under the control of Scer\GAL4Or47b.PC).

      (Root et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The loss of olfactory attraction behavior in response to an attractive odorant (apple cider vinegar) in adult flies expressing Lrp4KK101962 under the control of Scer\GAL4peb-GAL4 is ameliorated by co-expression of GABA-B-R2dsRNA.Scer\UAS while the decreased active zone number in olfactory receptor neurons is not suppressed.

      (Mosca et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GABA-B-R2RNAi.UAS
      GABA-B-R2dsRNA.Scer\UAS
      GABA-B-R2dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (8)