FlyBase Allele Report: Hsap\CELF1[UAS.cdHa]

General Information

Symbol

Hsap\CELF1^UAS.cdHa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of de Haro

FlyBase ID

FBal0279554

Feature type

allele

Associated gene

Hsap\CELF1

Associated Insertion(s)

Carried in Construct

P{UAS-CELF1.dH}

Also Known As

UAS-CUGBP1

Key Links

Transgenic product class

wild_type

(de Haro et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(de Haro et al., 2006)

Mutagen

in vitro construct

(de Haro et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-CELF1.dH}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\CELF1.

(de Haro et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\CELF1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Bernstein et al., 2016)

modeled by Hsap\MAPT^V337M.UAS

(Bernstein et al., 2016)

modeled by Hsap\MAPT^R406W.UAS

(Bernstein et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Hsap\CELF1^{Scer\UAS.cdHa} suppresses neurodegeneration in the Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} eye expression model of fragile X-associated tremor/ataxia syndrome.

(Sofola et al., 2007)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^GMR.PF

(Sofola et al., 2007)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Sofola et al., 2007)

eye, with Scer\GAL4^GMR.PU

(de Haro et al., 2013)

indirect flight muscle cell, with Scer\GAL4^Mhc.PW

(de Haro et al., 2006)

interommatidial bristle, with Scer\GAL4^GMR.PF

(de Haro et al., 2006)

Detailed Description

Statement

Reference

Scer\GAL4^GMR.PU-mediated expression results in mild ommatidial disorganization, loss of bristles and a reduction in size of the adult eye.

(de Haro et al., 2013)

High expression of Hsap\CELF1^{Scer\UAS.cdHa} under the control of Scer\GAL4^GMR.PF produces a rough eye phenotype.

(Sofola et al., 2007)

Flies expressing Hsap\CELF1^{Scer\UAS.cdHa} under the control of Scer\GAL4^GMR.PF exhibit inter-ommatidial bristle loss, but little if any ommatidial disorganisation.

Expression of Hsap\CELF1^{Scer\UAS.cdHa} in the indirect flight muscles under the control of Scer\GAL4^Mhc.PW results in vacuolisation and loss of muscle integrity.

(de Haro et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by Hrb87F^UAS.cSa, Scer\GAL4^GMR.PF

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by Hrb98DE^UAS.cSa, Scer\GAL4^GMR.PF

(Sofola et al., 2007)

Enhancer of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CTG^i480.UAS

(de Haro et al., 2006)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CTG^i480.UAS

(de Haro et al., 2006)

Suppressor of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

NOT Suppressor of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Hsap\HNRNPA2B1^UAS.cSa, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\HNRNPA2B1^UAS.cSa, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by smg[+]/smg¹

(de Haro et al., 2013)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^Mhc.PW has indirect flight muscle cell phenotype, enhanceable by Hsap\CELF1^UAS.cdHa, Scer\GAL4^Mhc.PW

(de Haro et al., 2006)

Suppressed by

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by smg^EP3556, Scer\GAL4^GMR.PU

(de Haro et al., 2013)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hrb87F^UAS.cSa, Scer\GAL4^GMR.PF

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hrb98DE^UAS.cSa, Scer\GAL4^GMR.PF

(Sofola et al., 2007)

Enhancer of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^Mhc.PW is an enhancer of indirect flight muscle cell phenotype of Hsap\CELF1^UAS.cdHa, Scer\GAL4^Mhc.PW

(de Haro et al., 2006)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CTG^i480.UAS

(de Haro et al., 2006)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CTG^i480.UAS

(de Haro et al., 2006)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^Mhc.PW is an enhancer of indirect flight muscle cell | conditional phenotype of Scer\GAL4^Mhc.PW, Zzzz\CTG^i480.UAS

(de Haro et al., 2006)

Suppressor of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

NOT Suppressor of

Statement

Reference

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\HNRNPA2B1^UAS.cSa, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Hsap\CELF1^UAS.cdHa, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Hsap\HNRNPA2B1^UAS.cSa, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CELF1^{Scer\UAS.cdHa} suppresses the fused ommatidia phenotype seen when Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Hrb87F^Scer\UAS.cSa suppresses the rough eye phenotype seen when Hsap\CELF1^{Scer\UAS.cdHa} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Hrb98DE^Scer\UAS.cSa suppresses the rough eye phenotype seen when Hsap\CELF1^{Scer\UAS.cdHa} is expressed under the control of Scer\GAL4^GMR.PF.

Co-expression of Hsap\CELF1^{Scer\UAS.cdHa} does not prevent Hsap\HNRNPA2B1^Scer\UAS.cSa from suppressing the fused ommatidia phenotype seen when Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(Sofola et al., 2007)

Expression of Hsap\CELF1^{Scer\UAS.cdHa} enhances the eye phenotypes seen when Zzzz\CTG^{i480.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. The amount of ommatidial disorganisation/fusion is increased.

Co-expression of Hsap\CELF1^{Scer\UAS.cdHa} and Zzzz\CTG^{i480.Scer\UAS} under the control of Scer\GAL4^Mhc.PW enhances the vacuolisation and loss of muscle integrity seen in the indirect flight muscles when either construct is expressed alone. Some muscle packs are absent and distribution of myofibrils is lax in the rest of the tissue. A dramatic loss of muscle structure is seen.

(de Haro et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\CELF1^{Scer\UAS.cdHa}

Hsap\CELF1^UAS.cdHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Haro

Secondary FlyBase IDs

References (4)

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