FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4ple.TH-C'
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General Information
Symbol
Scer\GAL4ple.TH-C'
Species
S. cerevisiae
Name
FlyBase ID
FBal0282525
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{ple-GAL4.TH-C'}
Also Known As
TH-C'-Gal4, THC'GAL4, TH-C', C'-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Liu et al., 2012)
Progenitor genotype
Scer\GAL4ple.PF
(Liu et al., 2012)
Carried in construct
P{ple-GAL4.TH-C'}
Cytology
Description

Deletion of part of the ple sequence.

(Liu et al., 2012)
Allele components
Component
Use(s)
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dopaminergic PAL neuron | subset
      (Liu et al., 2012)
      dopaminergic PPM2 neuron | subset
      (Pathak et al., 2015, Liu et al., 2012)
      dopaminergic PPL2ab neuron
      (Agrawal and Hasan, 2015, Liu et al., 2012)
      dopaminergic PPL1 neuron | subset
      (Pathak et al., 2015)
      adult dopaminergic PAM neuron | subset
      (Agrawal and Hasan, 2015, Pathak et al., 2015)
      adult dopaminergic VUM neuron of the gnathal ganglion | subset
      (Pathak et al., 2015)
      dopaminergic neuron | subset
      (Pathak et al., 2015)
      dopaminergic PAL neuron
      (Agrawal and Hasan, 2015)
      dopaminergic PPM1 neuron
      (Agrawal and Hasan, 2015)
      dopaminergic PPM2 neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of prothoracic neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of abdominal neuron
      (Agrawal and Hasan, 2015)
      (Agrawal and Hasan, 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4ple.TH-C' drives expression in a subset of ple-positive dopaminergic neurons in the adult brain as well as in the thorax and abdomen.

      (Pathak et al., 2015)

      ScerGAL4ple.TH-C' drives expression in 5 or fewer dopaminergic neurons in each cluster where it is expressed.

      (Agrawal and Hasan, 2015)

      ScerGAL4ple.TH-C' drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ScerGAL4ple.PF : around 3 PAL neurons, 5 PPM2 neurons and 6 PPL2 neurons. There was also some non-systematic expression in less than 1 neuron in the PAM and PPM1 clusters.

      (Liu et al., 2012)
       
      Marker for
      Reflects expression of
      ple
      (Liu et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of a subset of Scer\GAL4ple.TH-C' cells (activation restricted using Scer\GAL80ple.TH-F) in adults in combination with a paired odor, does not result in any significant short-term associative olfactory memory, compared to controls; activation of this subset specifically during odor-stimulus pairing and not other time intervals significantly increases aversive, but not appetitive, short-term associative olfactory memory, compared to controls, and this enhancement is lost when activation is further restricted using Scer\GAL80ple.PU.

      Inhibition (using shits.UAS.cUa at 32[o]C) of a subset of Scer\GAL4ple.TH-C' cells (activation restricted using Scer\GAL80ple.TH-F) in adults does not significantly affect short-term aversive of attractive associative olfactory memory, compared to controls.

      (Boto et al., 2019)

      Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4ple.TH-C' cells in adults does not affect startle-induced climbing behavior, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4ple.TH-C' cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

      (Sun et al., 2018)

      Inhibition (using shi1.UAS at 29[o]C) of Scer\GAL4ple.TH-C' cells in adults significantly reduces the number of animals capable of flight, compared to controls; animals that do not fly have significant wing coordination defects, compared to controls; there is also a significant reduction in the duration of electrophysiological spiking in dorsal longitudinal indirect flight muscles in response to an air puff, compared to controls.

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4ple.TH-C' cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces the number of animals capable of flight, compared to controls; there is also a significant reduction in the number of animals that display electrophysiological spiking in dorsal longitudinal indirect flight muscles in response to an air puff, compared to controls.

      (Sadaf et al., 2015)

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4ple.TH-C' cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect aversive shock or temperature olfactory memory, compared to controls.

      (Galili et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4ple.TH-C'
      Name Synonyms
      Secondary FlyBase IDs
        References (16)