FlyBase Allele Report: Ecol\lexA[GMR50A07]

General Information

Symbol

Ecol\lexA^GMR50A07

Species

E. coli

Name

FlyBase ID

FBal0289684

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{GMR50A07-lexA}

Also Known As

50A07-LexA

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2013.10.9)

Progenitor genotype

Carried in construct

P{GMR50A07-lexA}

Cytology

Description

A 3902 base pair fragment from the flanking non-coding or intronic region of mspo is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR50A07

Encoded product / tool

lexA::p65

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult mushroom body

(Vaccaro et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | adult stage, with TrpA1^lexAop.cBa

(Vaccaro et al., 2020)

decreased sleep | adult stage | heat sensitive, with TrpA1^lexAop.cBa

(Vaccaro et al., 2020)

increased cell death | adult stage, with TrpA1^lexAop.cBa

(Vaccaro et al., 2020)

short lived, with TrpA1^lexAop.cBa

(Vaccaro et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible | partially by Scer\GAL4^elav.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible | partially by Scer\GAL4^elav.PU/Sod2^UAS.cMa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has increased cell death | adult stage phenotype, suppressible by Scer\GAL4^mex1.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has abnormal stress response | adult stage phenotype, suppressible by Scer\GAL4^mex1.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has increased cell death | adult stage phenotype, suppressible by Scer\GAL4^Myo31DF.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has abnormal stress response | adult stage phenotype, suppressible by Scer\GAL4^Myo31DF.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Scer\GAL4^mex1.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Scer\GAL4^mex1.PU/Sod2^UAS.cMa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Scer\GAL4^Myo31DF.PU/Sod2^UAS.cMa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Scer\GAL4^Myo31DF.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Cat^UAS.cAa/Scer\GAL4^mex1.PU

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible by Cat^UAS.cAa/Scer\GAL4^Myo31DF.PU

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has short lived phenotype, suppressible | partially by Cat^UAS.cAa/Scer\GAL4^elav.PU

(Vaccaro et al., 2020)

NOT suppressed by

Statement

Reference

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has decreased sleep | adult stage | heat sensitive phenotype, non-suppressible by Cat^UAS.cAa/Scer\GAL4^elav.PU

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has decreased sleep | adult stage | heat sensitive phenotype, non-suppressible by Scer\GAL4^elav.PU/Sod1^UAS.cWa

(Vaccaro et al., 2020)

Ecol\lexA^GMR50A07, TrpA1^lexAop.cBa has decreased sleep | adult stage | heat sensitive phenotype, non-suppressible by Scer\GAL4^elav.PU/Sod2^UAS.cMa

(Vaccaro et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

53538

w¹¹¹⁸; P{GMR50A07-lexA}attP40

Notes on Origin

Discoverer

Comments

Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

(Pfeiffer et al., 2013.10.9)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ecol\lexA^GMR50A07

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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