In ptcD130, ptc16/ptcD130 and ptcD130/Df(2R)ED1742 mutant embryos, ganglionic branches do not extend into the ventral nerve cord. Defects in the dorsal branch, elongation and in dorsal trunk thickness and convolution are also observed. In addition, the visceral branches are very often misplaced, localising more ventrally than in the wild-type.
Tracheal development begins normally in ptcD130 mutant embryos. Placodes are correctly positioned and the first steps of cell migration occur normally. The initial steps of tracheal development are apparently regular until embryonic stage 12. At this point, the first defects in ganglionic branch extension are observed. In mutant stage 15 embryos, the anterior lateral trunk and ganglionic branch fail to separate. Despite this, ptcD130 mutant embryos display the same anterior and posterior lateral trunk/ganglionic branch extensions as in wild-type, by the end of embryogenesis.
From placode stages, ptcD130 mutants show a lower number of tracheal cells than wild-type embryos. On average, 63 cells are found in each tracheal metamere 5 in stage 13 embryos in ptcD130 mutants, in comparison with 97 cells in wild-type embryos.