FlyBase Allele Report: Dmel\kn[UAS.cHa.Tag:HA]

General Information

Symbol

Dmel\kn^{UAS.cHa.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0296596

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

P{UAS-kn.H.HA}

Also Known As

UAS-kn::HA

Key Links

Transgenic product class

cDNA

(Hattori et al., 2007)

wild_type

(Hattori et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hattori et al., 2007)

wild_type

(Hattori et al., 2007)

Mutagen

in vitro construct

(Hattori et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-kn.H.HA}

Cytology

Description

UASt regulatory sequences drive expression of kn cDNA clone RE03728 fused with three copies of Tag:HA.

(Hattori et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4²²¹

(Hattori et al., 2013)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4²²¹

(Hattori et al., 2007)

abnormal neuroanatomy | larval stage, with Scer\GAL4²²¹

(Hattori et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of kn^{Scer\UAS.cHa.T:Ivir\HA1} does not significantly affect the compartment organisation of Golgi in larval dendrites.

(Zhou et al., 2014)

The expression of kn^{Scer\UAS.cHa.T:Ivir\HA1} under the control of Scer\GAL4²²¹ results in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons exhibiting a significant increase in the number of dendrite branch terminals, as compared to controls.

(Hattori et al., 2013)

Expression of kn^{Scer\UAS.cHa.T:Ivir\HA1} via Scer\GAL4^2-21 increases the number of higher order branches in class I neurons. kn^{Scer\UAS.cHa.T:Ivir\HA1} mis-expression transforms dendritic trees of class I into those of class IV-like in terms of branching complexity and continuous higher-order branch formation, but not in terms of the field size of the avoidance behaviour.

Expression of Scer\GAL4^smid-C161>kn^{Scer\UAS.cHa.T:Ivir\HA1} causes class II neurons to acquire exuberant branches. They develop far more complicated dendritic arbors than the control branches in terms of both number and total length. On the other hand, Scer\GAL4^smid-C161>kn^{Scer\UAS.cHa.T:Ivir\HA1} expression greatly decreases the number of terminal structures that distinguish class III. Short straight "spikes" typically cover long branches of the wild-type class III dendrite. In contrast, branches in the mutants are almost naked with only a small number of longer and curved protrusions.

(Hattori et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, suppressible by CG14642^GD6058, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, suppressible by Pak3^GD8481, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, suppressible by lola^KK110256/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, suppressible by Orai^GD3554, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, suppressible by Imp^GD9232, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4²²¹/ab^UAS.L.cSa

(Hattori et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abnormal neuroanatomy phenotype, non-suppressible by Ten-m^GD3620/Scer\GAL4²²¹

(Hattori et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, suppressible by lola^KK110256/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, suppressible by lola^KK110256/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, suppressible by lola^KK110256/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, suppressible by Orai^GD3554, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, suppressible by Orai^GD3554, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, suppressible by Orai^GD3554, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, suppressible by Imp^GD9232, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, suppressible by Imp^GD9232, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, suppressible by Imp^GD9232, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, suppressible by CG14642^GD6058, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, suppressible by CG14642^GD6058, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, suppressible by CG14642^GD6058, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, suppressible by Pak3^GD8481, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, suppressible by Pak3^GD8481, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, suppressible by Pak3^GD8481, Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | larval stage phenotype, suppressible by Scer\GAL4²²¹/ab^UAS.L.cSa

(Hattori et al., 2007)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has larval multidendritic class I neuron | larval stage phenotype, suppressible by Scer\GAL4²²¹/ab^UAS.L.cSa

(Hattori et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype, non-suppressible by Ten-m^GD3620/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype, non-suppressible by Ten-m^GD3620/Scer\GAL4²²¹

(Hattori et al., 2013)

Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA} has dendritic tree | third instar larval stage phenotype, non-suppressible by Ten-m^GD3620/Scer\GAL4²²¹

(Hattori et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of dendrite terminals in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons expressing kn^{Scer\UAS.cHa.T:Ivir\HA1} under the control of Scer\GAL4²²¹ is fully suppressed by co-expressing either lola^KK110256 or olf186-F^GD3554, is partially suppressed by co-expressing either Imp^GD9232, CG14642^GD6058 or Pak3^GD8481, but is not suppressed by co-expressing Ten-m^GD3620.

(Hattori et al., 2013)

Co-expression of ab^{Scer\UAS.L.cSa} reduces the length of 3rd- and higher-order branches compared with expression of Scer\GAL4^2-21>kn^{Scer\UAS.cHa.T:Ivir\HA1} alone.

(Hattori et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kn^{Scer\UAS.cHa.T:Ivir\HA1}

kn^{UAS.cHa.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (4)

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