FlyBase Allele Report: Dmel\aurB[35.33]

General Information

Symbol

Dmel\aurB^35.33

Species

D. melanogaster

Name

FlyBase ID

FBal0301282

Feature type

allele

Associated gene

Dmel\aurB

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Das et al., 2016, Mathieu et al., 2013)

Progenitor genotype

Cytology

Description

Amino acid replacement: L166F.

(Das et al., 2016, Mathieu et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,857,427..10,857,427 [-]

Nucleotide change:

C10857427T

Amino acid change:

L166F | aurB-PA

Reported amino acid change:

L166F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Das et al., 2016, Mathieu et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | third instar larval stage (with aurB¹⁶⁸⁹)

(Gallaud et al., 2022)

lethal

(Mathieu et al., 2013)

Phenotype Manifest In

germarium | germline clone

(Mathieu et al., 2013)

germline cell | germline clone

(Mathieu et al., 2013)

larval neuroblast | third instar larval stage (with aurB¹⁶⁸⁹)

(Gallaud et al., 2022)

nucleus | germline clone

(Mathieu et al., 2013)

nurse cell spectrosome | germline clone

(Mathieu et al., 2013)

Detailed Description

Statement

Reference

Neuroblasts in aurB^35.33/aurB¹⁶⁸⁹ transheterozygous third instar larval brains show increased time in mitosis

(Gallaud et al., 2022)

aurB^35.33 mutant germline clones exhibit defects in early germ-cell development, with the formation of a giant germline stem cell that fills the entire germarium. These mutant cells display highly polyploid nuclei, enlarged spectrosomes and do not come out of the germarium.

(Mathieu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^35.33 is an enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

aurB^35.33/aurB[+] is a suppressor of abnormal cytokinesis | dominant phenotype of shrb^G5

(Matias et al., 2015)

aurB^35.33/aurB[+] is a suppressor | partially of abnormal cell cycle phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^35.33 is an enhancer of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

aurB^35.33/aurB[+] is a suppressor of female germline stem cell phenotype of shrb^G5

(Matias et al., 2015)

aurB^35.33/aurB[+] is a suppressor of egg chamber phenotype of shrb^G5

(Matias et al., 2015)

aurB^35.33/aurB[+] is a suppressor of female germline cell phenotype of shrb^G5

(Matias et al., 2015)

aurB^35.33/aurB[+] is a suppressor | partially of egg chamber phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^35.33/aurB[+] is a suppressor | partially of ring canal phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^35.33/aurB[+] is a suppressor | partially of nurse cell phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^35.33/aurB[+] is a suppressor | partially of oocyte phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^35.33/aurB[+] is a suppressor | partially of female germline cyst phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^35.33/aurB[+] is a suppressor | partially of female fusome phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The fraction of egg chambers containing 32 cells and the fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is reduced in aurB^35.33/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

(Matias et al., 2015)

A aurB^35.33/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Mathieu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

aurB^L27

(Taniguchi et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

35.33

(Mathieu et al., 2013)

aurB^35.33

(Taniguchi et al., 2018, Das et al., 2016)

aurB³⁵³³

(Matias et al., 2015)

ial^35.33

(Gallaud et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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