FlyBase Allele Report: Dmel\Det[S125E.UASp.GFP]

General Information

Symbol

Dmel\Det^{S125E.UASp.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0301295

Feature type

allele

Associated gene

Dmel\Det

Associated Insertion(s)

Carried in Construct

P{UASp-Det-GFP.S125E}

Key Links

Transgenic product class

missense_variant

(Mathieu et al., 2013)

polypeptide_post_translational_processing_variant

(Mathieu et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mathieu et al., 2013)

polypeptide_post_translational_processing_variant

(Mathieu et al., 2013)

Mutagen

in vitro construct

(Mathieu et al., 2013)

Progenitor genotype

Carried in construct

P{UASp-Det-GFP.S125E}

Cytology

Description

Amino acid replacement: S125E.

(Mathieu et al., 2013)

UASp regulatory sequences drive expression of a mutated Det coding region fused at the C-terminal with GFP. This transgene contains an amino acid residue mutation that mimics constitutive phosphorylation of Det at the aurB consensus site.

(Mathieu et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Det

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Phenotype Manifest In

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female fusome, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

nurse cell, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

ring canal, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Detailed Description

Statement

Reference

Overexpression of Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in a significant increase in both the number of germline cells per cyst and the penetrance of the phenotype. Almost 50% of mutant egg chambers exhibit 32 cells or more, with oocytes displaying up to 6 ring canals.

Expression of Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} in dividing germline cysts, using the Scer\GAL4^{bam.T:Hsim\VP16} driver, which is expressed from cystoblasts to eight cell cysts but not in germline stem cells, does not result in any extra germline cells.

Live-imaging of germline stem cells expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} reveals the germline stem cells to be dividing synchronously with several neighboring germline cells, suggesting that they may be physically connecting, which is never seen in a wild-type situation. The spectrosomes in these germline stem cells are branched as fusomes and linked germline stem cells with up to eight other germline cells. These synchronous cells appear to have characteristics of both stem cells and cyst cells. They do not grow indefinitely, and the fusome eventually breaks down, indicating that Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} delays abscission, but does not block it completely.

(Mathieu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Suppressed by

Statement

Reference

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, enhanceable by CycB[+]/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, enhanceable by CycB^KG08886/CycB²

(Mathieu et al., 2013)

Suppressed by

Statement

Reference

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, suppressible | partially by aurB^2A43/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, suppressible | partially by aurB^35.33/aurB[+]

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female germline cyst phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has female fusome phenotype, suppressible by hts⁰¹¹⁰³/hts¹

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has egg chamber phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has ring canal phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR} has oocyte phenotype, suppressible | partially by Incenp[+]/Incenp^QA26

(Mathieu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

A hts¹/hts⁰¹¹⁰³ background, which removes the fusome from ovaries, results in suppression of the synchronous additional division found in germline stem cells expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Reducing the level of CycB, through a CycB²/+ background, greatly enhances the Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} gain-of-function phenotype. Further reduction in the level of CycB, through a CycB²/CycB^KG08886 background, greatly enhances the Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} gain-of-function phenotype.

A Incenp^QA26/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

A aurB^2A43/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

A aurB^35.33/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Mathieu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Det^{S125E.UASp.GFP} is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Det²¹⁸⁰

(Mathieu et al., 2013)

Comments

A Det²¹⁸⁰/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Mathieu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Det^{S125E.Scer\UAS.P\T.T:Avic\GFP}

Det^{S125E.UASp.GFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

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