Phalloidin staining of hts01103 mutant neuromuscular junctions (NMJs) in third instar larvae reveal an actin distribution distinct from wild-type. hts01103 mutants display abnormal actin rings throughout the muscle that are not observed in wild-type.
mtDNA replication (EdU incorporation) is completely absent in region 2b of hts01103/Df(2R)BSC26 mutant germaria. Replication appears normal in the ovarian stem cells and post-germarium egg chambers.
hts01103/Df(2R)BSC26 flies do not show any lesions in the lamina or medulla one day after eclosion. However, vacuoles develop in the lamina after 14 days, and extend to the medulla after 28 days. Wild type brains of this age are normal.
Fourteen-day-old hts01103/Df(2R)BSC26 flies show severe deficits in climbing up a tube (8cm) within 8 seconds, compared to wild type.
20 day old hts01103/Df(2R)BSC26 males show elevated spindle misorientation in the male germline stem cells (GSCs), without a significant increase in centrosome misorientation compared to controls. Spindle misorientation is not seen in the GSCs of newly eclosed males.
hts01103/Df(2R)BSC26 flies show a severe disruption of the medulla. The regular array of R7 and R8 axons is lost and axons clump together forming irregularly spaced thick bundles and gaps in between. R8 and R7 axons follow disordered paths from M1 to their respective target layer and specifically R8 often overshoots its correct target layer.
Flies that are overall hts01103/+ but have hts01103 homozygous eyes show no obvious defect in the medulla.
Many egg chambers from hts01103/Df(2R)BSC26 frequently lack a full complement of germ cells.
Gonial cells of hts01103/Df(2R)BSC26 testes do not show fusome structures by immunostaining of Klp61F.
In contrast to wild type, many spermatocytes in hts01103/Df(2R)BSC26 mutants contain too many or too few centrosomes.
In contrast to wild type, 33% of meiotic spermatocytes in hts01103/Df(2R)BSC26 mutant testes show spindle abnormalities, including monopolar and multipolar spindles.
While spermatocytes within a cyst proceed through meiosis in near synchrony in wild type animals, cysts of hts01103/Df(2R)BSC26 testes harbour spermatocytes at several different stages of meiosis as well as post-meiotic spermatids.
visible rough eye, pale ocelli, bent wing