FlyBase Allele Report: Dmel\Nhe2[UAS.cGa]

General Information

Symbol

Dmel\Nhe2^UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0304401

Feature type

allele

Associated gene

Dmel\Nhe2

Associated Insertion(s)

Carried in Construct

P{UAS-Nhe2.G}

Also Known As

UAS-Dnhe2

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Grillo-Hill et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-Nhe2.G}

Cytology

Description

UASt regulatory sequences drive expression of a Nhe2 cDNA. The cDNA is essentially identical to the C-isoform (FBpp0289186), with the following polymorphisms: A157T, T283I, insL568, missing exon 17 (72 amino acids), 12 additional amino acids at the C terminus (SKGEFQHTGGRY).

(Grillo-Hill et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nhe2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number, with Scer\GAL4^en.PU

(Grillo-Hill et al., 2015)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

increased occurrence of cell division | pupal stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

visible, with Scer\GAL4^ey.3.5.Exel

(Grillo-Hill et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

Phenotype Manifest In

basal stalk, with Scer\GAL4^109-30

(Ulmschneider et al., 2016)

cone cell | pupal stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

eye, with Scer\GAL4^ey.3.5.Exel

(Grillo-Hill et al., 2015)

eye, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

eye disc posterior to the morphogenetic furrow, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

eye photoreceptor cell, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

follicle cell, with Scer\GAL4^109-30

(Ulmschneider et al., 2016)

germarium, with Scer\GAL4^109-30

(Ulmschneider et al., 2016)

ommatidium, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

ommatidium | pupal stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

primary pigment cell, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

retina | pupal stage, with Scer\GAL4^GMR.PU

(Grillo-Hill et al., 2015)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Grillo-Hill et al., 2015)

Detailed Description

Statement

Reference

The expression of Nhe2^UAS.cGa under the control of Scer\GAL4^109-30 leads to ovarioles frequently exhibiting multilayered stalks (but no apparent defect in stalk cell differentiation) and leads to a significant increase in intracellular pH in pre-follicle and follicle cells within the germarium, as compared to controls.

(Ulmschneider et al., 2016)

Expression of Nhe2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU induces an extremely rough adult eye and phenotypes consistent with dysplasia of the underlying epithelium. Ommatidial organization is disrupted and individual facets are irregular in shape and size. Misoriented ommatidia, abnormal cell fates and missing R cells are also seen. Expressing Nhe2^Scer\UAS.cGa earlier in eye development under the control of Scer\GAL4^ey.3.5.Exel causes a very mild rough eye phenotype.

The intracellular pH (pHi) in larval eye discs expressing Nhe2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU is similar to controls. The pHi is significantly higher compared to wild type in pupal eyes at 42 hours after pupal formation, in both apical non-neural cells and in photoreceptor neurons.

The eyes of pupae expressing Nhe2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU have severely disrupted cell shapes and tissue organization, and ommatidia overall are smaller then in wild type. Ommatidia have aberrant numbers of cone and primary pigment cells. Some ommatidia are fused. Filamentous actin levels are lower in the cone cells and lattice cells, but reduced in the retina overall. The number and arrangement of cone cell and R cell nuclei are aberrant. There is a significant increase of proliferating cells posterior to the morphogenetic furrow in third instar larvae (the second mitotic wave).

Expression of Nhe2^Scer\UAS.cGa in the posterior compartment of the developing wing disc under the control of Scer\GAL4^en.PU results in overgrowth and dysplasia in the expressing compartment. There is a significant increase in the number of proliferating cells in the posterior compartment compared to the anterior compartment.

(Grillo-Hill et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Nhe2^UAS.cGa, Scer\GAL4^109-30 is a suppressor | partially of basal stalk phenotype of Scer\GAL4^109-30, ptc^JF03223

(Ulmschneider et al., 2016)

Other

Statement

Reference

Ae2[+]/Ae2¹, Nhe2^UAS.cGa, Scer\GAL4^109-30 has egg chamber phenotype

(Benitez et al., 2019)

Ae2[+]/Ae2¹, Nhe2^UAS.cGa, Scer\GAL4^109-30 has ovariole phenotype

(Benitez et al., 2019)

Nhe2^UAS.cGa, Ras85D^V12.UAS, Scer\GAL4^ptc.PU has wing disc phenotype

(Grillo-Hill et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing Nhe2^UAS.cGa under the control of Scer\GAL4^109-30 in a Ae2¹ heterozygous background leads to significantly fewer mid-stage follicles/egg chambers (i.e. between the germarium and late stages), as compared to controls.

(Benitez et al., 2019)

The co-expression of Nhe2^UAS.cGa partially suppresses the basal stalk multilayering observed upon the expression of ptc^JF03223 under the control of Scer\GAL4^109-30.

(Ulmschneider et al., 2016)

Co-expression of Nhe2^Scer\UAS.cGa and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^ptc.PU results in a significant increase in the overall size of the wing disc compared with either transgene alone or wild type. There is also an increase in the number of invasive cells seen.

(Grillo-Hill et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Mef2.PR/Nhe2^UAS.cGa rescues Nhe2^null

(Grillo-Hill et al., 2015)

Fails to rescue

Scer\GAL4^elav.PU/Nhe2^UAS.cGa fails to rescue Nhe2^null

(Grillo-Hill et al., 2015)

Comments

The semi-lethality of Nhe2^null flies is rescued by expression of Nhe2^Scer\UAS.cGa using the muscle-specific Scer\GAL4^Mef2.PR driver, but not using the neuronal Scer\GAL4^elav.PU driver.

(Grillo-Hill et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nhe2^Scer\UAS.cGa

Nhe2^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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