FlyBase Allele Report: Dmel\Tcs3[1A2]

General Information

Symbol

Dmel\Tcs3^1A2

Species

D. melanogaster

Name

FlyBase ID

FBal0318696

Feature type

allele

Associated gene

Dmel\Tcs3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lin et al., 2015)

Progenitor genotype

Cytology

Description

Amino acid replacement: D201N.

(Lin et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,352,715..16,352,715 [+]

Nucleotide change:

G16352715A

Amino acid change:

D201N | Tcs3-PA

Reported amino acid change:

D201N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lin et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

decreased body size | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

lethal - all die before end of P-stage (with Df(3L)ED220)

(Lin et al., 2015)

melanotic mass phenotype (with Df(3L)ED220)

(Lin et al., 2015)

Phenotype Manifest In

embryonic/larval hemocyte | second instar larval stage (with Df(3L)ED220)

(Lin et al., 2015)

embryonic/larval lymph gland | second instar larval stage (with Df(3L)ED220)

(Lin et al., 2015)

lamellocyte | second instar larval stage | increased number (with Df(3L)ED220)

(Lin et al., 2015)

melanotic mass (with Df(3L)ED220)

(Lin et al., 2015)

Detailed Description

Statement

Reference

Compared to wild type (pupate around day 5, eclose around day 10), Tcs3^1A2/Df(3L)ED220 exhibit an extended larval period prior to lethality (viability: 97% at day 8, 30% at day 16) and have melanotic masses ('black spots': 50% at day 8, 100% at day 16); larvae are slim.

Compared to control Tcs3^1A2/+ heterozygote larval hemolymph (made up of primarily plasmatocytes and more rare lamellocytes), Tcs3^1A2/Df(3L)ED220 hemolymph from second instar larvae shows overrepresentation of lamellocytes; compared to Tcs3^1A13/Df(3L)ED220, Tcs3^1A2/Df(3L)ED220 have similar hematopoietic phenotypes but they are weaker and/or slower to develop.

(Lin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Lin et al., 2015)

(Lin et al., 2015)

(Lin et al., 2015)

(Lin et al., 2015)

(Lin et al., 2015)

(Lin et al., 2015)

Rescued by

Df(3L)ED220/Tcs3^1A2 is rescued by Tcs3^GFP(S65T)

(Lin et al., 2015)

Df(3L)ED220/Tcs3^1A2 is rescued by Tcs3^+tLa

(Lin et al., 2015)

Comments

Tcs3^{T:Avic\GFP-S65T} or Tcs3^+tLa rescues Tcs3^1A2/Df(3L)ED220 to adult viability and no melanotic masses are seen in rescued animals.

(Lin et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The Tcs3^1A2 and Tcs3^1A19 mutant lines are independent isolations of the same lesion in Tcs3 (amino acid replacement D201N) as they were isolated from different chromosomes.

(Lin et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

1A2

(Lin et al., 2015)

Tcs3^1A2

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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