FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4Tmc.3.0
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General Information
Symbol
Scer\GAL4Tmc.3.0
Species
S. cerevisiae
Name
FlyBase ID
FBal0322948
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Tmc-GAL4.3.0}
Also Known As
Tmc-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Zhang et al., 2016)
Progenitor genotype
Carried in construct
P{Tmc-GAL4.3.0}
Cytology
Description

3.0kb of genomic sequence from upstream of the predicted Tmc transcription start site regulates expression of a GAL4 driver.

(Zhang et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
Tmc
Tmc_tmc-3kb
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      labellar multidendritic neuron
      (Zhang et al., 2016)
      multidendritic neuron of ventral cibarial sense organ | subset
      (Zhang et al., 2016)
      adult subesophageal zone | restricted
      (Zhang et al., 2016)
      adult antennal lobe projection neuron vPN
      (Zhang et al., 2016)
      Kenyon cell | subset
      (Zhang et al., 2016)
      neuron of prothoracic coxa-trochanter joint
      (Zhang et al., 2016)
      neuron of mesothoracic coxa-trochanter joint
      (Zhang et al., 2016)
      neuron of metathoracic coxa-trochanter joint
      (Zhang et al., 2016)
      adult ventral nerve cord | subset
      (Zhang et al., 2016)
      labellar multidendritic neuron
      (Zhang et al., 2016)
      ventral cibarial sense organ neuron
      (Zhang et al., 2016)
      neuron of wing hinge
      (Zhang et al., 2016)
      neuron of adult ventral nerve cord
      (Zhang et al., 2016)
      neuron of leg

      Comment: NOT tarsal leg segment

      (Zhang et al., 2016)
      antennal lobe projection neuron vPN
      (Zhang et al., 2016)
      neuron of adult mushroom body
      (Zhang et al., 2016)
      neuron of adult subesophageal zone
      (Zhang et al., 2016)
      Additional Information
      Statement
      Reference

      ScerGAL4Tmc.3.0 is expressed in a labellar multidendritic neuron in each of the two bilaterally symmetrical labella. The cell body of these neurons is adjacent to the I6 sensilla at the proximal part of the labella, while the dendritic branches innervate the sensilla, predominately, but not exclusively, the L-type sensilla.

      (Zhang et al., 2016)

      Expression of ScerGAL4Tmc.3.0 in the adult antennal lobe projection neurons and Kenyon cells may be ectopic rather than representative of Tmc.

      (Zhang et al., 2016)
       
      Marker for
      Reflects expression of
      Tmc
      (Bloomington Drosophila Stock Center, 2017, Zhang et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of Scer\GAL4Tmc.3.0 cells in adult females significantly increases preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls; inhibition does not affect preference for 100 mM sucrose-containing (rather than plain) agarose for egg laying, compared to controls; increased preference for softer 100 mM sucrose-containing agarose is still seen when inhibition is restricted to a subset of Scer\GAL4Tmc.3.0 cells using Scer\GAL80VGlut.PV.

      Inhibition (using Ctet\tetXTNT-LC.UAS) of Scer\GAL4Tmc.3.0 cells in adult females significantly increases preference for softer 100 mM sucrose-containing agarose for egg laying, compared to controls.

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with red light) of a subset of Scer\GAL4Tmc.3.0 cells (restricted using Scer\GAL80VGlut.PV) in adult females significantly decreases egg laying, compared to baseline.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4Tmc.3.0 cells in adult females significantly decreases egg laying, compared to baseline.

      (Wu et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Tmc.3.0
      Name Synonyms
      Secondary FlyBase IDs
        References (11)