FlyBase Allele Report: Dmel\kn[UAS.cSa.Tag:HA]

General Information

Symbol

Dmel\kn^{UAS.cSa.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0327177

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

PBac{UAS-kn.HA}

Also Known As

UAS-col-HA

Key Links

Transgenic product class

wild_type

(Stratmann et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Stratmann et al., 2016)

Mutagen

in vitro construct

(Stratmann et al., 2016)

Progenitor genotype

Carried in construct

PBac{UAS-kn.HA}

Cytology

Description

UAS regulatory sequences drive expression of kn, tagged with Tag:HA.

(Stratmann et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | late embryonic stage, with Scer\GAL4^lbe.K

(Stratmann et al., 2019)

abnormal neuroanatomy | late embryonic stage, with Scer\GAL4^wg-MD758

(Stratmann et al., 2019)

Phenotype Manifest In

abdominal neuroblast NB5-6 | late embryonic stage, with Scer\GAL4^lbe.K

(Stratmann et al., 2019)

medial longitudinal fascicle | late embryonic stage, with Scer\GAL4^wg-MD758

(Stratmann et al., 2019)

Detailed Description

Statement

Reference

Expressing kn^{UAS.cSa.Tag:HA} under the control of Scer\GAL4^wg-MD758 results in a significant increase in axons in the medial-most Fas2-immunopositive fascicle of the embryonic ventral nerve cord.

Expressing kn^{UAS.cSa.Tag:HA} under the control of Scer\GAL4^lbe.K leads to the axonal misrouting of neurons derived from abdominal neuroblast NB5-6 towards the medial-most Fas2-immunopositive fascicle of the embryonic ventral nerve cord.

(Stratmann et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^lbe.K, kn^{UAS.cSa.Tag:HA} has abnormal neuroanatomy | late embryonic stage phenotype, non-suppressible by ap^P44/ap^P44

(Stratmann et al., 2019)

Scer\GAL4^lbe.K, kn^{UAS.cSa.Tag:HA} has abnormal neuroanatomy | late embryonic stage phenotype, non-suppressible by Df(2L)BSC354/eya^cli-IID

(Stratmann et al., 2019)

Other

Statement

Reference

Scer\GAL4^elav-C155, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has abnormal neuroanatomy | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^wg-MD758, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has abnormal neuroanatomy | late embryonic stage phenotype

(Stratmann et al., 2019)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^lbe.K, kn^{UAS.cSa.Tag:HA} has abdominal neuroblast NB5-6 | late embryonic stage phenotype, non-suppressible by ap^P44/ap^P44

(Stratmann et al., 2019)

Scer\GAL4^lbe.K, kn^{UAS.cSa.Tag:HA} has abdominal neuroblast NB5-6 | late embryonic stage phenotype, non-suppressible by Df(2L)BSC354/eya^cli-IID

(Stratmann et al., 2019)

Other

Statement

Reference

Scer\GAL4^elav-C155, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has apterous-expressing neuron of the lateral cluster of the thorax | ectopic | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^elav-C155, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has medial longitudinal fascicle | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^elav-C155, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has lateral longitudinal fascicle | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^wg-MD758, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has apterous-expressing neuron of the lateral cluster of the thorax | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^wg-MD758, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has medial longitudinal fascicle | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^wg-MD758, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has lateral longitudinal fascicle | late embryonic stage phenotype

(Stratmann et al., 2019)

Scer\GAL4^wg-MD758, kn^{UAS.cSa.Tag:HA}, lbe^UAS.Tag:MYC has embryonic/larval motor neuron | late embryonic stage phenotype

(Stratmann et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing lbe^UAS.Tag:MYC and kn^{UAS.cSa.Tag:HA} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^wg-MD758 in embryos results in the formation of ectopic ap-expressing neurons which project axons into a common medial fascicle. This in turn leads to the loss of lateral longitudinal fascicles and an overall reduction of the number of longitudinal fascicles. Co-expression under the control of Scer\GAL4^wg-MD758 results in a loss of motor neuron projections in thoracic nerves.

(Stratmann et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

83309

y¹ w¹¹¹⁸; PBac{UAS-kn.HA}VK00033/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kn^{Scer\UAS.cSa.T:Ivir\HA1}

kn^{UAS.cSa.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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