FlyBase Allele Report: Dmel\Ptp69D[CRISPR.14]

General Information

Symbol

Dmel\Ptp69D^CRISPR.14

Species

D. melanogaster

Name

FlyBase ID

FBal0336316

Feature type

allele

Associated gene

Dmel\Ptp69D

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Poe et al., 2017)

Progenitor genotype

Cytology

Description

Frame-shifting mutation i the first extracellular Ig domain: 8bp deletion starting in codon 116 plus insertion of TATCCA in place of the sequence AATTCC normally found at codons 123 and 124.

(Poe et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:12,736,399..12,736,406 [+]

Comment:

An 8 bp deletion causes a frameshift after Q115. In addition, 14 bp downstream of the deletion, the sequence AATTCC is replaced with TATCCA.

(Poe et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone | third instar larval stage

(Poe et al., 2017)

abnormal neuroanatomy | third instar larval stage (with Df(3L)8ex34)

(Poe et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

MARCM clones for Ptp69D^CRISPR.14 in class IV dendritic arborizing neurons in third instar larvae show strong reduction in terminal dendrite density (but not total dendrite density or terminal dendrite number) compared to controls. Ptp69D^CRISPR.14/Df(3L)8ex34 transheterozygous larvae display severe decrease in both the total and terminal dendrite density as well as terminal dendrite number.

(Poe et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ptp69D^CRISPR.14/Df(3L)8ex34 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by sotv^GD2025/Scer\GAL4^A58

(Poe et al., 2017)

Enhancer of

Statement

Reference

Ptp69D^CRISPR.14/Df(3L)8ex34 is an enhancer of abnormal neuroanatomy | third instar larval stage | cell non-autonomous phenotype of Scer\GAL4^A58, sotv^GD2025

(Poe et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ptp69D^CRISPR.14/Df(3L)8ex34 has larval multidendritic class IV neuron | third instar larval stage phenotype, enhanceable by sotv^GD2025/Scer\GAL4^A58

(Poe et al., 2017)

Ptp69D^CRISPR.14/Df(3L)8ex34 has dendritic tree | third instar larval stage phenotype, enhanceable by sotv^GD2025/Scer\GAL4^A58

(Poe et al., 2017)

Ptp69D^CRISPR.14/Df(3L)8ex34 has distal dendrite | third instar larval stage phenotype, enhanceable by sotv^GD2025/Scer\GAL4^A58

(Poe et al., 2017)

Enhancer of

Statement

Reference

Ptp69D^CRISPR.14/Df(3L)8ex34 is an enhancer of larval multidendritic class IV neuron | third instar larval stage | cell non-autonomous phenotype of Scer\GAL4^A58, sotv^GD2025

(Poe et al., 2017)

Ptp69D^CRISPR.14/Df(3L)8ex34 is an enhancer of dendritic tree | third instar larval stage | cell non-autonomous phenotype of Scer\GAL4^A58, sotv^GD2025

(Poe et al., 2017)

Ptp69D^CRISPR.14/Df(3L)8ex34 is an enhancer of distal dendrite | third instar larval stage | cell non-autonomous phenotype of Scer\GAL4^A58, sotv^GD2025

(Poe et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased terminal dendrite density and number in class IV dendritic arborizing neurons observed in third instar larvae either expressing sotv^GD2025 (with the Scer\GAL4^A58 driver) or transheterozygous for Ptp69D^CRISPR.14/Df(3L)8ex34 is further enhanced in the double mutants (Scer\GAL4^A58>sotv^GD2025;Ptp69D^CRISPR.14/Df(3L)8ex34).

(Poe et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ptp69D^CRISPR.14/Df(3L)8ex34 is rescued by Scer\GAL4^ppk.PU/Ptp69D^UAS.Tag:MYC

(Poe et al., 2017)

Ptp69D^CRISPR.14/Df(3L)8ex34 is rescued by Ptp69D^ΔIg.UAS/Scer\GAL4^ppk.PU

(Poe et al., 2017)

Comments

The strong decrease in the total and terminal dendrite density as well as terminal dendrite number observed in class IV dendrite arborizing neurons in Ptp69D^CRISPR.14/Df(3L)8ex34 third instar larvae is fully rescued by expression of either Ptp69D^{Scer\UAS.T:Hsap\MYC} or Ptp69D^{ΔIg.Scer\UAS} under the control of Scer\GAL4^ppk.PU in the mutant background.

(Poe et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97519

w^*; wg^Sp-1/CyO, P{Wee-P.ph0}2; Ptp69D^CRISPR.14 P{neoFRT}80B P{neoFRT}82B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ptp69D¹⁴

(Poe et al., 2017)

Ptp69D^CRISPR.14

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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