FlyBase Allele Report: Scer\GAL4[Uba5-CR00497-TG4.2]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^{Uba5-CR00497-TG4.2}

General Information

Symbol

Scer\GAL4^{Uba5-CR00497-TG4.2}

Species

S. cerevisiae

Name

FlyBase ID

FBal0340756

Feature type

allele

Associated gene

Associated Insertion(s)

TI{CRIMIC.TG4.2}Uba5^{CR00497-TG4.2}

Carried in Construct

Genomic Maps

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.2}Uba5^{CR00497-TG4.2}

Cytology

Description

A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Uba5, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CATATTTGGAGTGCTAGCTGTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 1bp of genomic sequence flanking the insertion site.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.2}

RMCE target element

Encoded product / tool

binary expression system - driver

Also carries

integrase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

X:11,483,974..11,483,975 [+]

(Pan et al., 2023, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval glial cell | subset

(Pan et al., 2023)

larval neuron | subset

(Pan et al., 2023)

larval central nervous system | restricted

(Pan et al., 2023)

adult neuron | subset

(Pan et al., 2023)

adult glial cell | subset

(Pan et al., 2023)

adult brain | restricted

(Pan et al., 2023)

Additional Information

Statement

Reference

Expression is observed in a subset of neurons and glia in both larval CNS and adult brain. Uba5 is expressed more widely in the adult brains than in the larval CNS. In larval CNS, it is expressed in many fewer neurons than elav.

(Pan et al., 2023)

Marker for

Reflects expression of

(Pan et al., 2023)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\UBA5^D389G.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Scer\GAL4^{Uba5-CR00497-TG4.2}/Hsap\UBA5^A371T.UAS is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^Q312L.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^V260M.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^Y53F.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^M57V.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of majority die during embryonic stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of some die during first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Scer\GAL4^{Uba5-CR00497-TG4.2}/Hsap\UBA5^R72C.UAS is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Scer\GAL4^{Uba5-CR00497-TG4.2}/Hsap\UBA5^D389Y.UAS is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

NOT Suppressor of

Statement

Reference

Hsap\UBA5^C250A.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a non-suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^L254P.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a non-suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Scer\GAL4^{Uba5-CR00497-TG4.2}/Hsap\UBA5^C303R.UAS is a non-suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^G168E.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a non-suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^R55H.UAS/Scer\GAL4^{Uba5-CR00497-TG4.2} is a non-suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

78928

y¹ w^* TI{CRIMIC.TG4.2}Uba5^{CR00497-TG4.2}/FM7h

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{Uba5-CR00497-TG4.2}

Uba5^T2A-Gal4

(Pan et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections