FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\NPFRsk8
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General Information
Symbol
Dmel\NPFRsk8
Species
D. melanogaster
Name
FlyBase ID
FBal0343404
Feature type
allele
Associated gene
Dmel\NPFR
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ameku et al., 2018)
Progenitor genotype
Cytology
Description

Mutation within the NPFR coding sequence; deletion of 8 nucleotides.

(Ameku et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:6,192,117..6,192,124 [+]
Comment:

Deletion of 8bp starting in codon 30 of NPFR causes a frameshift and early translation termination.

(Ameku et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      NPFRsk8/Df(3R)BSC464 transheterozygous females fail to show the expected mating-induced (and NFPF-induced) increase in germline stem cells in the germarium, as compared to heterozygous controls; the expression of NPFRUAS.cYa under the control of Scer\GAL4NP1624 rescues the germline stem cell number in mated females, but also leads to an abnormal increase in germline stem cells in virgin females.

      (Ameku et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      NPFRsk8, dpphr56/dpp[+] has decreased cell number | oogenesis | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, sax[+]/sax5 has decreased cell number | oogenesis | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, tkv1/tkv[+] has decreased cell number | oogenesis | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, put[+]/put135 has decreased cell number | oogenesis | conditional phenotype

      (Ameku et al., 2018)
      Phenotype Manifest In
      Other
      Statement
      Reference

      NPFRsk8, dpphr56/dpp[+] has female germline stem cell | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, sax[+]/sax5 has female germline stem cell | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, tkv1/tkv[+] has female germline stem cell | conditional phenotype

      (Ameku et al., 2018)

      NPFRsk8, put[+]/put135 has female germline stem cell | conditional phenotype

      (Ameku et al., 2018)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      NPFRsk8,dpphr56, NPFRsk8,sax5, NPFRsk8,tkv1 or NPFRsk8,put135 double heterozygous females fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to heterozygous controls.

      (Ameku et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(3R)BSC464/NPFRsk8 is rescued by NPFRUAS.cYa/Scer\GAL4Akh.PL

      (Yoshinari et al., 2021)
      Partially rescued by

      Df(3R)BSC464/NPFRsk8 is partially rescued by NPFRUAS.cYa/Scer\GAL4NP1624

      (Ameku et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NPFR8
      (Gao et al., 2024)
      NPFRsk8
      (Orozco Ramirez et al., 2025, Yoshinari et al., 2021, Ameku et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)