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General Information
Symbol
Dmel\αTub84BK40A
Species
D. melanogaster
Name
FlyBase ID
FBal0345033
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Comment:

The endogenous AAG (lysine) codon was substituted with an GCG (alanine) codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

αTub84B sequence which carries the K40A amino acid replacement has been knocked in to the attP site present in the αTub84BKO-attP knockout line.

Insertion components
TI{TI}αTub84BK40A
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

αTub84BK40A mutant larvae show significantly decreased responses (rolling) to gentle and harsh touch (at 44mN), and to sound, but not to noxious heat (39.5[o]C), as compared to controls. αTub84BK40A mutant larvae, however, do not exhibit obvious locomotion defects.

In αTub84BK40A mutant larvae, C3da neuron dendrites present significantly more microtubules (Futsch immunostaining) than controls.

αTub84BK40A larval class IV da neurons do not show apparent changes to the polarized dendritic localization of Golgi outposts ( ManII::GFP-labelled ). Retrograde transport of lysosomes in dendrites exhibits a significant decrease in speed but a significant increase in flux, whereas the anterograde transport is not affected, as compared to controls; lysosome transport in axons (both anterograde and retrograde) is not affected. Although the overall dendritic coverage appears normal, there is a progressive increase in the number of terminal branches (increase observed at 120h AEL, but not at 48h AEL), as compared to controls; dendrites show a significantly higher frequency of extension events, despite of no change in de novo branch growth or in retraction events, as compared to controls. There is a severe decrease in growing microtubules (EB1 comets) in dendrites, but not in axons, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
NOT Enhancer of
Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)