FlyBase Allele Report: Dmel\Abi[5]

General Information

Symbol

Dmel\Abi⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0350332

Feature type

allele

Associated gene

Dmel\Abi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kim et al., 2019)

Mutagen

P-element activity

(Kim et al., 2019)

Progenitor genotype

P{EP}Abi^G6718

(Kim et al., 2019)

Cytology

Description

1075bp deletion that removes a large portion of both the second and third exons of Abi. Generated by imprecise excision of P{EP}Abi^G6718.

(Kim et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

abnormal neurophysiology | third instar larval stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

abnormal neurophysiology | third instar larval stage (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^Abi.PK

(Kim et al., 2019)

lethal - all die during P-stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

majority die during P-stage (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^elav-C155

(Kim et al., 2019)

majority die during P-stage (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^how-24B

(Kim et al., 2019)

partially lethal - majority die (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^elav-C155

(Kim et al., 2019)

partially lethal - majority die (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^how-24B

(Kim et al., 2019)

partially lethal - majority live (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^elav-C155, Scer\GAL4^how-24B

(Kim et al., 2019)

some die during P-stage (with Df(3R)su(Hw)7), with Abi^UAS.Tag:HA, Scer\GAL4^elav-C155, Scer\GAL4^how-24B

(Kim et al., 2019)

uncoordinated (with Df(3R)su(Hw)7)

(Kim et al., 2019)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

microtubule | third instar larval stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

NMJ bouton | third instar larval stage (with Df(3R)su(Hw)7)

(Kim et al., 2019)

Detailed Description

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 third instar larvae display NMJ overgrowth with supernumerary boutons (both mature and satellite), increased evoked excitatory junction potential amplitudes and uncoordinated motor behavior, with a faster roll-over than controls in a behavioral assay. A significant increase in the number of synaptic boutons containing microtubule loops points towards an increase in microtubule stability.

When expressing Abi^UAS.Tag:HA under the control of Scer\GAL4^Abi.PK in Abi⁵/Df(3R)su(Hw)7 third instar larvae, evoked excitatory junction potential amplitudes decrease significantly, below control levels.

Bouton numbers are unaffected in Abi⁵/+ larvae.

(Kim et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wit^B11

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wit^A12

(Kim et al., 2019)

NOT suppressed by

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abl^UAS.cFa

(Kim et al., 2019)

Suppressor of

Statement

Reference

Abi⁵/Abi[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

Abi⁵/Abi[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Abl^UAS.cFa, Scer\GAL4^elav-C155

(Kim et al., 2019)

NOT Suppressor of

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of wit^B11/wit^A12

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Fmr1^UAS.cZa, Scer\GAL4^elav-C155

(Kim et al., 2019)

Other

Statement

Reference

Abi⁵, SCAR^Δ37/SCAR[+] has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Hem[+]/Hem^J4-48 has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Fmr1^Δ50M has abnormal neuroanatomy | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Abl¹/Abl[+] has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Abl⁴/Abl[+] has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Rac1^J11/Rac1[+] has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Kim et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wit^A12

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wit^B11

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 has NMJ bouton | third instar larval stage phenotype, suppressible by wit^A12

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 has NMJ bouton | third instar larval stage phenotype, suppressible by wit^B11

(Kim et al., 2019)

NOT suppressed by

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abl^UAS.cFa

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abl^UAS.cFa

(Kim et al., 2019)

Suppressor of

Statement

Reference

Abi⁵/Abi[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

Abi⁵/Abi[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

Abi⁵/Abi[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Abl^UAS.cFa, Scer\GAL4^elav-C155

(Kim et al., 2019)

Abi⁵/Abi[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of Abl^UAS.cFa, Scer\GAL4^elav-C155

(Kim et al., 2019)

NOT Suppressor of

Statement

Reference

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of wit^B11/wit^A12

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of wit^B11/wit^A12

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^UAS.cZa, Scer\GAL4^elav-C155

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Fmr1^UAS.cZa, Scer\GAL4^elav-C155

(Kim et al., 2019)

Other

Statement

Reference

Abi⁵, Abl¹/Abl[+] has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Abl¹/Abl[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, SCAR^Δ37/SCAR[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Hem[+]/Hem^J4-48 has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Hem[+]/Hem^J4-48 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Fmr1^Δ50M has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Fmr1^Δ50M has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Abl⁴/Abl[+] has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Abl⁴/Abl[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Rac1^J11/Rac1[+] has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, Rac1^J11/Rac1[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Abi⁵, SCAR^Δ37/SCAR[+] has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Abi⁵/Df(3R)su(Hw)7 is rescued by Scer\GAL4^Abi.PK/Abi^UAS.Tag:HA

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is rescued by Abi^UAS.Tag:HA/Scer\GAL4^da.G32

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is rescued by Scer\GAL4^elav-C155/Abi^{W452K.UAS.Tag:HA}

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is rescued by Scer\GAL4^elav-C155/Abi^{4YE.UAS.Tag:HA}

(Kim et al., 2019)

Partially rescued by

Abi⁵/Df(3R)su(Hw)7 is partially rescued by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is partially rescued by Abi^UAS.Tag:HA/Scer\GAL4^how-24B

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is partially rescued by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA/Scer\GAL4^how-24B

(Kim et al., 2019)

Not rescued by

Abi⁵/Df(3R)su(Hw)7 is not rescued by Scer\GAL4^elav-C155/Abi^{Δ30-65.UAS.Tag:HA}

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is not rescued by Scer\GAL4^elav-C155/Abi^{Δ123-175.UAS.Tag:HA}

(Kim et al., 2019)

Abi⁵/Df(3R)su(Hw)7 is not rescued by Scer\GAL4^elav-C155/Abi^{4YF.UAS.Tag:HA}

(Kim et al., 2019)

Comments

Expressing Abi^UAS.Tag:HA under the control of either Scer\GAL4^Abi.PK or Scer\GAL4^da.G32 fully rescues pupal lethality of Abi⁵/Df(3R)su(Hw)7 flies, while expression under the control of Scer\GAL4^elav-C155, Scer\GAL4^how-24B or under the combined control of Scer\GAL4^elav-C155 and Scer\GAL4^how-24B results in only a partial rescue.

Expressing Abi^UAS.Tag:HA under the control of Scer\GAL4^elav-C155 (but not Scer\GAL4^how-24B) rescues the supernumerary phenotype of both mature and satellite boutons at the NMNJ and the uncoordinated phenotype of Abi⁵/Df(3R)su(Hw)7 third instar larvae in a roll over assay.

The supernumerary phenotype of mature and satellite boutons at the NMNJ of Abi⁵/Df(3R)su(Hw)7 third instar larvae is rescued by expressing Abi^{W452K.UAS.Tag:HA} or Abi^{4YE.UAS.Tag:HA}, but not Abi^{Δ30-65.UAS.Tag:HA}, Abi^{Δ123-175.UAS.Tag:HA} or Abi^{4YF.UAS.Tag:HA} under the control of Scer\GAL4^elav-C155.

(Kim et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97206

w^*; Abi⁵/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Abi⁵

abi⁵

(Park et al., 2022, Kim et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (2)

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