FlyBase Allele Report: Dmel\Gpdh1[B18]

General Information

Symbol

Dmel\Gpdh1^B18

Species

D. melanogaster

Name

FlyBase ID

FBal0353204

Feature type

allele

Associated gene

Dmel\Gpdh1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Li et al., 2019)

Mutagen

CRISPR/Cas9

(Li et al., 2019)

Progenitor genotype

Cytology

Description

7bp (ACGTGTT) deletion within exon 4; frameshift mutation affects the C-terminal catalytic domain.

(Li et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:5,946,679..5,946,685 [+]

Comment:

A 7bp deletion in Gpdh1 causes a frameshift and early translation termination.

(Li et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larva (with Gpdh1^A10)

(Li et al., 2019)

Detailed Description

Statement

Reference

Gpdh1^A10/Gpdh1^B18 larvae do not show major developmental defects, despite a small but significant decrease in body mass at second instar.

(Li et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Gpdh1^A10/Gpdh1^B18 is an enhancer of increased mortality during development phenotype of Ldh¹⁷/Ldh¹⁶

(Li et al., 2019)

Other

Statement

Reference

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has abnormal developmental rate | second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has decreased body size | second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has abnormal size | second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has some die during first instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has lethal - all die before end of second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has majority die during second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has visible | second instar larval stage phenotype

(Li et al., 2019)

Phenotype Manifest In

Other

Statement

Reference

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has larval brain | second instar larval stage phenotype

(Li et al., 2019)

Gpdh1^A10/Gpdh1^B18, Ldh¹⁷/Ldh¹⁶ has embryonic/larval posterior midgut | second instar larval stage phenotype

(Li et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Ldh¹⁶/Ldh¹⁷, Gpdh1^A10/Gpdh1^B18 larvae show severe developmental defects: second instar larvae are much smaller and have a much lower body weight, as compared to either single transheterozygous condition and wild-type controls; 92-96h after egg-laying, larvae are still at L2, whereas either single transheterozygous condition and wild-type controls are already at L3; at L2 brains are much smaller and, even in size-matched larvae, presents significantly lower mitotic index, as compared to either single transheterozygous condition and wild-type controls; L2 posterior midguts are also much smaller than either single transheterozygous condition and wild-type controls.

(Li et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

94703

w¹¹¹⁸; Gpdh1^B18/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Gpdh1^B18

(Li et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close