PRL-1¹⁶ mutants develop into morphologically normal adults. Upon treatment of flies with various stress stimuli, including ultraviolet, X-ray, cold, heat, starvation, CO[[2]], N[[2]] or ether, only high concentration of CO[[2]] treatment (5L/min CO[[2]] for 20s) causes a highly prevalent, non-reversible held-up wing phenotype; this phenotype is mostly induced at 3 days post eclosion onwards; spontaneous held-up wings are uncommon in the absence of CO[[2]] exposure; PRL-1¹⁶ mutants with held-up wings have the same lifespan as controls; PRL-1¹⁶ mutants exhibit the same avoidance to CO[[2]] as controls.

Examination of PRL-1¹⁶ mutant indirect flight muscles by phalloidin staining and transmission electrical microscopy shows no obvious differences to controls.