FlyBase Allele Report: Dmel\sov[EA42]

General Information

Symbol

Dmel\sov^EA42

Species

D. melanogaster

Name

FlyBase ID

FBal0356465

Feature type

allele

Associated gene

Dmel\sov

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Benner et al., 2019)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

G to A mutation at position 8140 relative to the first transcription start site of sov. This introduces a stop codon in place of amino acid residue Trp1437.

(Benner et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:6,764,462..6,764,462 [+]

Reported nucleotide change:

G6764462A

Amino acid change:

W1437term | sov-PA; W1437term | sov-PB; W1437term | sov-PC

Reported amino acid change:

W1437term

Comment:

Nonsense mutation (TGG to TAG) at Trp codon.

(Benner et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis (with sov²)

(Benner et al., 2019)

decreased cell number | germline clone | oogenesis

(Benner et al., 2019)

female sterile

(Wayne et al., 1995)

female sterile (with sov²)

(Benner et al., 2019)

female sterile (with sov^KG00226)

(Benner et al., 2019)

female sterile (with sov^NP6070)

(Benner et al., 2019)

lethal (with Df(1)sov)

(Benner et al., 2019)

lethal (with sov^ML150)

(Benner et al., 2019)

lethal | recessive

(Benner et al., 2019, Wayne et al., 1995)

male fertile

(Wayne et al., 1995)

neoplasia | oogenesis (with sov²)

(Benner et al., 2019)

partially lethal (with sov^KG00226)

(Benner et al., 2019)

partially lethal (with sov^NP6070)

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-2

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-12

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}118E-3

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}118E-10

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}Dyrk3^118E-15

(Benner et al., 2019)

Phenotype Manifest In

egg chamber (with sov²)

(Benner et al., 2019)

egg chamber | germline clone

(Benner et al., 2019)

germline cyst

(Wayne et al., 1995)

ovariole

(Wayne et al., 1995)

ovariole (with sov²)

(Benner et al., 2019)

ovary (with sov²)

(Benner et al., 2019)

spectrosome (with sov²)

(Benner et al., 2019)

Detailed Description

Statement

Reference

The ovaries of sov²/sov^EA42 transheterozygous females show a range of phenotypes, including abnormal numbers of spectrosomes in the germarium, degenerate tissue characterized by small, nub-like ovaries comprising dysmorphic germaria, ovarian tumors and empty ovarioles.

sov^EA42 female germline clones do not produce vitellogenic egg chambers or mature eggs.

(Benner et al., 2019)

Homozygous females produce small ovaries that contain few egg cysts and disorganised ovarioles. The egg cysts that develop have aberrant morphology, including abnormal numbers of nurse cells and patches of necrotic cells.

(Wayne et al., 1995)

Lethality acts at the embryonic and larval stages. abnormal oogenesis

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sov^EA42 has lethal phenotype, suppressible by Dp(1;3)DC486

(Benner et al., 2019)

sov^EA42 has decreased cell number | germline clone | oogenesis phenotype, suppressible by Dp(1;3)sn^13a1

(Benner et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

sov^EA42 has egg chamber | germline clone phenotype, suppressible by Dp(1;3)sn^13a1

(Benner et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Df(1)sov female germline clones do not produce vitellogenic egg chambers or mature eggs, which is rescued by Dp(1;3)DC486.

(Benner et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sov^A1304-1

(Hammond et al., 2020)

Rescued by

sov^EA42 is rescued by sov^FSTF-sfGFP

(Benner et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

91359

sov^EA42 P{neoFRT}19A; Dp(1;3)DC486, PBac{DC486}VK00033

Notes on Origin

Discoverer

Lefevre.

In contrast to FBrf0080470, which suggested that the 'EA42' chromosome carries separable mutations in adjacent female-sterile and lethal loci, rescue of both the female sterility and lethality of the 'EA42' chromosome by duplications and a transgene containing sov suggests that the female sterility and lethality of the chromosome map to the same locus.

(Benner et al., 2019)

Separable from: sov⁴.

(Wayne et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(1)6Dc³

(Hammond et al., 2020)

l(1)EA42

(Wayne et al., 1995)

l(1)EM25

sov⁴

sov^EA42

(Hammond et al., 2020, Benner et al., 2019)

Name Synonyms

Secondary FlyBase IDs

FBal0046509
FBal0007068

References (4)

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