FlyBase Allele Report: Dmel\sov[ML150]

General Information

Symbol

Dmel\sov^ML150

Species

D. melanogaster

Name

FlyBase ID

FBal0046510

Feature type

allele

Associated gene

Dmel\sov

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Benner et al., 2019, Wayne et al., 1995)

Mutagen

ethyl methanesulfonate

(Wayne et al., 1995)

Progenitor genotype

Cytology

Description

C to G mutation at position 7566 relative to the first transcription start site of sov. This results in the amino acid replacement Q1246E.

(Benner et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:6,763,888..6,763,888 [+]

Nucleotide change:

C6763888G

Amino acid change:

Q1246E | sov-PA; Q1246E | sov-PB; Q1246E | sov-PC

Reported amino acid change:

Q1246E

(Benner et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis (with sov²)

(Benner et al., 2019)

abnormal size | adult stage | female (with sov²)

(Jankovics et al., 2018)

abnormal size | oogenesis (with sov²)

(Benner et al., 2019)

decreased cell number | oogenesis (with sov²)

(Jankovics et al., 2018)

female sterile (with sov²)

(Benner et al., 2019, Jankovics et al., 2018)

female sterile (with sov^KG00226)

(Benner et al., 2019)

female sterile (with sov^NP6070)

(Benner et al., 2019)

increased cell death | oogenesis (with sov²)

(Jankovics et al., 2018)

lethal (with Df(1)sov)

(Benner et al., 2019)

lethal (with sov^EA42)

(Benner et al., 2019)

lethal | recessive

(Benner et al., 2019, Wayne et al., 1995)

neoplasia | oogenesis (with sov²)

(Benner et al., 2019, Jankovics et al., 2018)

partially lethal (with sov²)

(Benner et al., 2019)

partially lethal (with sov^KG00226)

(Benner et al., 2019)

partially lethal (with sov^NP6070)

(Benner et al., 2019)

suppressor of variegation | dominant, with In(1)w^m4h

(Jankovics et al., 2018)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-2

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-12

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}118E-3

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}118E-10

(Benner et al., 2019)

suppressor of variegation | dominant, with P{hsp26-pt-T}Dyrk3^118E-15

(Benner et al., 2019)

Phenotype Manifest In

cystoblast (with sov²)

(Jankovics et al., 2018)

escort cell (with sov²)

(Jankovics et al., 2018)

female germline stem cell (with sov²)

(Jankovics et al., 2018)

germarium (with sov²)

(Jankovics et al., 2018)

ovary | adult stage (with sov²)

(Jankovics et al., 2018)

spectrosome (with sov²)

(Benner et al., 2019)

Detailed Description

Statement

Reference

The ovaries of sov²/sov^ML150 transheterozygous females progressive show a range of phenotypes, including abnormal numbers of spectrosomes in the germarium, degenerate tissue characterized by small, nub-like ovaries comprising dysmorphic germaria, ovarian tumors and empty ovarioles. sov^ML150 heterozygotes are viable and fertile.

(Benner et al., 2019)

sov²/sov^ML150 transheterozygous females exhibit rudimentary ovaries; while most germaria show no germ cells, almost all remaining germaria show germ cell tumors, with each cell containing a single spectrosome. Escort cells are decreased in number and are virtual absent from the niche.

Germaria in sov²/sov^ML150 transheterozygotes also show an increase in apoptotic cells, as compared to controls.

(Jankovics et al., 2018)

Homozygous female sterile sov mutations, sov¹, sov² or sov³, made heterozygous with sov^ML150 are viable but the ovarian phenotype becomes more severe.

(Wayne et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sov^ML150 has lethal phenotype, suppressible by Dp(1;3)sn^13a1

(Benner et al., 2019)

sov^ML150/sov² has decreased cell number | oogenesis phenotype, suppressible | partially by bam^hs.PO