FlyBase Allele Report: Dmel\Mecr[CR70041-TG4.1]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Mecr^{CR70041-TG4.1}

General Information

Symbol

Dmel\Mecr^{CR70041-TG4.1}

Species

D. melanogaster

Name

FlyBase ID

FBal0368475

Feature type

allele

Associated gene

Associated Insertion(s)

TI{CRIMIC.TG4.1}Mecr^{CR70041-TG4.1}

Carried in Construct

Key Links

Genomic Maps

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Dutta et al., 2023)

Mutagen

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.1}Mecr^{CR70041-TG4.1}

Cytology

Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG16935, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TATGCAGTTCATGCCGGTTATGG. The PCR check gave the expected-sized band for the right flank and no band for the left flank.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.1}

RMCE target element

Encoded product / tool

binary expression system - driver

Also carries

integrase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

2R:13,861,557..13,861,558 [-]

(Kanca et al., 2022, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of childhood-onset dystonia with optic atrophy and basal ganglia abnormalities

CEC

(Dutta et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval muscle system | larval stage

(Dutta et al., 2023)

mitochondrion | larval stage

(Dutta et al., 2023)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mecr^{CR70041-TG4.1} has increased mortality during development phenotype, suppressible by Scer\GAL4^da.G32/Hsap\MECR^UAS.Tag:HA

(Dutta et al., 2023)

Mecr^{CR70041-TG4.1} has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^da.G32/Hsap\MECR^{Gly232Glu.UAS}

(Dutta et al., 2023)

Mecr^{CR70041-TG4.1} has increased mortality during development phenotype, suppressible | partially by Hsap\MECR^{Arg258Trp.UAS}/Scer\GAL4^da.G32

(Dutta et al., 2023)

NOT suppressed by

Statement

Reference

Mecr^{CR70041-TG4.1} has lethal - all die before end of larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\MECR^{Tyr285Stop.UAS}

(Dutta et al., 2023)

Mecr^{CR70041-TG4.1} has some die during third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\MECR^{Tyr285Stop.UAS}

(Dutta et al., 2023)

Mecr^{CR70041-TG4.1} has some die during second instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\MECR^{Tyr285Stop.UAS}

(Dutta et al., 2023)

Other

Statement

Reference

Hsap\MECR^{Arg258Trp.UAS}, Mecr^{CR70041-TG4.1}, Scer\GAL4^da.G32 has decreased rate of adult locomotory behavior phenotype

(Dutta et al., 2023)

Hsap\MECR^{Gly232Glu.UAS}, Mecr^{CR70041-TG4.1}, Scer\GAL4^da.G32 has decreased rate of adult locomotory behavior phenotype

(Dutta et al., 2023)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mecr^{CR70041-TG4.1} is rescued by Mecr^GFP

(Dutta et al., 2023)

Mecr^{CR70041-TG4.1} is rescued by Mecr^UAS.Tag:HA/Scer\GAL4^da.G32

(Dutta et al., 2023)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

92694

y¹ w^*; TI{CRIMIC.TG4.1}Mecr^{CR70041-TG4.1}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG16935^{CR70041-TG4.1}

Mecr^{CR70041-TG4.1}

mecr^TG4

(Dutta et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections