UASt regulatory sequences drive expression of a wild-type Hsap\TNPO2 cDNA (GenBank:NM_001136196.1). The open reading frame (ORF) is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
UASt regulatory sequences drive expression of the Hsap\TNPO2 open reading frame, tagged at the C-terminal end with three copies of Tag:HA. The ORF was amplified from the OHS1770-202312693 clone. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
The expression of Hsap\TNPO2UAS.Tag:HA under the control of Scer\GAL4ey.PH or Scer\GAL4GMR.PF induces a small, rough eye.
Expression under the control of Scer\GAL4nub.PK induces smaller wings, with blisters and increased vein material.
Hsap\TNPO2UAS.Tag:HA/Scer\GAL4Tnpo-CR92235-TG4.2 is an enhancer of increased mortality during development phenotype of TnpoCR92235-TG4.2/TnpoG736D