FlyBase Allele Report: Dmel\Tnpo[CR92235-TG4.2]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Tnpo^{CR92235-TG4.2}

General Information

Symbol

Dmel\Tnpo^{CR92235-TG4.2}

Species

D. melanogaster

Name

FlyBase ID

FBal0364177

Feature type

allele

Associated gene

Associated Insertion(s)

TI{CRIMIC.TG4.2}Tnpo^{CR92235-TG4.2}

Carried in Construct

Key Links

Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Goodman et al., 2021)

Mutagen

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.2}Tnpo^{CR92235-TG4.2}

Cytology

Description

A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Tnpo, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CAAGCGTAATTTAAGAGTAATGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 12bp of genomic sequence flanking the insertion site.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.2}

RMCE target element

Encoded product / tool

binary expression system - driver

Also carries

integrase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

3L:6,197,189..6,197,201 [+]

(Goodman et al., 2021, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual developmental disorder with hypotonia, impaired speech, and dysmorphic facies

CEC

(Goodman et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

(Goodman et al., 2021)

lethal - all die before end of larval stage (with Df(3L)Exel8101)

(Goodman et al., 2021)

lethal - all die before end of larval stage (with Tnpo^G736D)

(Goodman et al., 2021)

lethal - all die before end of larval stage (with Tnpo^Δ11)

(Goodman et al., 2021)

some die during second instar larval stage (with Df(3L)Exel8101)

(Goodman et al., 2021)

some die during third instar larval stage (with Df(3L)Exel8101)

(Goodman et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, enhanceable by Hsap\TNPO2^UAS.Tag:HA/Scer\GAL4^{Tnpo-CR92235-TG4.2}

(Goodman et al., 2021)

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, enhanceable by Hsap\TNPO2^{Q28R.UAS.Tag:HA}/Scer\GAL4^{Tnpo-CR92235-TG4.2}

(Goodman et al., 2021)

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, enhanceable by Scer\GAL4^{Tnpo-CR92235-TG4.2}/Hsap\TNPO2^{D156N.UAS.Tag:HA}

(Goodman et al., 2021)

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, enhanceable by Scer\GAL4^{Tnpo-CR92235-TG4.2}/Hsap\TNPO2^{W727C.UAS.Tag:HA}

(Goodman et al., 2021)

NOT Enhanced by

Statement

Reference

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, non-enhanceable by Hsap\TNPO2^{W370C.UAS.Tag:HA}/Scer\GAL4^{Tnpo-CR92235-TG4.2}

(Goodman et al., 2021)

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, non-enhanceable by Scer\GAL4^{Tnpo-CR92235-TG4.2}/Hsap\TNPO2^{A546V.UAS.Tag:HA}

(Goodman et al., 2021)

NOT suppressed by

Statement

Reference

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, non-suppressible by Hsap\TNPO2^{W370C.UAS.Tag:HA}/Scer\GAL4^{Tnpo-CR92235-TG4.2}

(Goodman et al., 2021)

Tnpo^{CR92235-TG4.2}/Tnpo^G736D has increased mortality during development phenotype, non-suppressible by Scer\GAL4^{Tnpo-CR92235-TG4.2}/Hsap\TNPO2^{A546V.UAS.Tag:HA}

(Goodman et al., 2021)

Other

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tnpo^{CR92235-TG4.2} is rescued by Tnpo^GR

(Goodman et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

91480

y¹ w^*; TI{CRIMIC.TG4.2}Tnpo^{CR92235-TG4.2}/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tnpo^{CR92235-TG4.2}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections