FlyBase Allele Report: Zzzz\VHH[deGradFP.UAS]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Zzzz\VHH^deGradFP.UAS

General Information

Symbol

Zzzz\VHH^deGradFP.UAS

Species

a. artificial

Name

FlyBase ID

FBal0401141

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

M{UAS-deGradFP}

P{UAS-deGradFP}

Also Known As

UAS-Nslmb-vhhGFP4, UAS-deGradFP, deGradFP

Transgenic product class

inframe_deletion

(Caussinus et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Caussinus et al., 2011)

Mutagen

in vitro construct

(Caussinus et al., 2011)

Progenitor genotype

Carried in construct

M{UAS-deGradFP}

P{UAS-deGradFP}

Cytology

Description

UASt regulatory sequences drive expression of deGradFP.

(Caussinus et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

gene product degradation tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodevelopmental disorder with regression, abnormal movements, loss of speech, and seizures

CEC with Pits^{MI02926-DH.PT-GFSTF.1}

(Marcogliese et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | heat sensitive, with Pde4^{MI03415-GFSTF.2}, Scer\GAL4^117Y

(Nagarkar-Jaiswal et al., 2015)

abnormal locomotor behavior | adult stage, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^Act5C.PI

(Marcogliese et al., 2022)

abnormal locomotor behavior | adult stage | progressive, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^Act5C.PI

(Marcogliese et al., 2022)

abnormal locomotor behavior | adult stage | progressive, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

abnormal locomotor behavior | heat sensitive, with Scer\GAL4^nSyb.PS, brp^{MI02987-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

abnormal memory | conditional, with Scer\GAL4^Mef2.247, meng^{MI03008-GFSTF.1}

(Lee et al., 2018)

abnormal neuroanatomy | adult stage | progressive, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

abnormal neurophysiology, with Scer\GAL4^ey.PU, brp^{MI02987-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

abnormal neurophysiology | heat sensitive, with Scer\GAL4^nSyb.PS, brp^{MI02987-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

abnormal planar polarity, with Rab23^EYFP, Scer\GAL4^en.PU

(Dunst et al., 2015)

fertile, with Scer\GAL4^nullo.PG

(Caussinus et al., 2011)

fertile, with Scer\GAL4^Tub.PU

(Caussinus et al., 2011)

lethal | heat sensitive, with Scer\GAL4^da.PU, brp^{MI02987-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

lethal | heat sensitive, with Scer\GAL4^nSyb.PS, brp^{MI02987-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^Tub.PU, dlg1^{MI06353-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

lethal - all die during embryonic stage | heat sensitive, with α-Cat^{MI02577-GFSTF.0}, Scer\GAL4^Act.PU

(Nagarkar-Jaiswal et al., 2015)

short lived, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^Act5C.PI

(Marcogliese et al., 2022)

short lived, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

viable, with Scer\GAL4^nullo.PG

(Caussinus et al., 2011)

viable, with Scer\GAL4^Tub.PU

(Caussinus et al., 2011)

visible, with α-Cat^{MI02577-GFSTF.0}, Scer\GAL4^ey.PU

(Nagarkar-Jaiswal et al., 2015)

Phenotype Manifest In

axon | adult stage | decreased number, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^Act5C.PI

(Marcogliese et al., 2022)

axon | adult stage | decreased number, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

embryonic/larval midgut | heat sensitive, with Scer\GAL4^Tub.PU, dlg1^{MI06353-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

embryonic/larval neuromuscular junction | heat sensitive, with Scer\GAL4^Tub.PU, dlg1^{MI06353-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

eye, with α-Cat^{MI02577-GFSTF.0}, Scer\GAL4^ey.PU

(Nagarkar-Jaiswal et al., 2015)

larval brain | heat sensitive, with Scer\GAL4^Tub.PU, dlg1^{MI06353-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

marginal nerve, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^Act5C.PI

(Marcogliese et al., 2022)

marginal nerve, with Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

wing disc | heat sensitive, with Scer\GAL4^Tub.PU, dlg1^{MI06353-GFSTF.0}

(Nagarkar-Jaiswal et al., 2015)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS, Zzzz\VHH^deGradFP.UAS has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by CkIα^JF01792, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

Pits^{MI02926-DH.PT-GFSTF.1}, Scer\GAL4^nSyb.PS, Zzzz\VHH^deGradFP.UAS has short lived phenotype, suppressible | partially by CkIα^JF01792, Scer\GAL4^nSyb.PS

(Marcogliese et al., 2022)

Other

Statement

Reference

HDAC4^CA07134, Zzzz\VHH^deGradFP.UAS has abnormal neuroanatomy | adult stage phenotype

(Tan et al., 2024)

HDAC4^{ΔANK.UAS.Tag:MYC}, HDAC4^CA07134, Scer\GAL4^ey-OK107, Zzzz\VHH^deGradFP.UAS has abnormal neuroanatomy | adult stage phenotype

(Tan et al., 2024)

HDAC4^{3SA.UAS.Tag:MYC}, HDAC4^CA07134, Scer\GAL4^ey-OK107, Zzzz\VHH^deGradFP.UAS has abnormal neuroanatomy | adult stage phenotype

(Tan et al., 2024)

Dif^B-GFP, Scer\GAL4^αTub84B.PL, Zzzz\VHH^deGradFP.UAS has abnormal chemosensory behavior | adult stage phenotype

(Wijesekera et al., 2022)

Phenotype Manifest In

Other

Statement

Reference

HDAC4^CA07134, Zzzz\VHH^deGradFP.UAS has lobe system of adult mushroom body phenotype

(Tan et al., 2024)

HDAC4^{ΔANK.UAS.Tag:MYC}, HDAC4^CA07134, Scer\GAL4^ey-OK107, Zzzz\VHH^deGradFP.UAS has lobe system of adult mushroom body phenotype

(Tan et al., 2024)

HDAC4^{3SA.UAS.Tag:MYC}, HDAC4^CA07134, Scer\GAL4^ey-OK107, Zzzz\VHH^deGradFP.UAS has lobe system of adult mushroom body phenotype

(Tan et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

sqh^AX3 embryos which carry sqh^{RLC.T:Avic\GFP-S65T} and which also express slmb^{Scer\UAS.T:Zzzz\vhhGFP4} under the control of Scer\GAL4^332.3 in the postmitotic amnioserosa cells show a dorsal open phenotype.

Df(2R)BSC696 flies carrying ap^{KI.T:Avic\GFP} and also expressing slmb^{Scer\UAS.T:Zzzz\vhhGFP4} under the control of Scer\GAL4^tub.PU have wing defects.

(Caussinus et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

38421

w^*; P{UAS-deGradFP}3

38422

y¹ w^*; P{UAS-deGradFP}2

38417

w^*; P{nullo-GAL4.G}5.20; P{UAS-deGradFP}3

38420

w^*; P{en2.4-GAL4}e16E, P{UAS-mCherry.NLS}2; P{UAS-deGradFP}3/TM3, Sb¹

38418

w^*; P{GawB}nub-AC-62, P{UAS-mCherry.NLS}2; P{UAS-deGradFP}3/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Comments

slmb^{Scer\UAS.T:Zzzz\vhhGFP4} can be used to cause the degradation of proteins that are tagged with T:Avic\GFP and T:Avic\GFP^EYFP.

(Caussinus et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

UAS_NSlmb-vhh-GFP4

(Ell et al., 2024)

Zzzz\VHH^deGradFP.UAS

deGradFP

(Ell et al., 2024)

slmb^{Scer\UAS.T:Zzzz\vhhGFP4}

slmb^{Scer\UAS.attB.T:Zzzz\vhhGFP4}

slmb^UAS.vhhGFP4

Name Synonyms

Secondary FlyBase IDs

FBal0400744
FBal0298957
FBal0267307
FBal0267308

References (29)

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