FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Experimental tool: deGradFP
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General Information
Symbol
deGradFP
FlyBase ID
FBto0000943
Name
Description
Description

deGradFP is an engineered F-box protein that targets GFP-tagged proteins for degradation via the ubiquitin pathway. deGradFP contains the N-terminal 198 amino acids of D. melanogaster slmb (this includes the F-box domain), fused to VHH(GFP4), a nanobody that binds GFP (and some of its derivatives, including YFP, EYFP and Venus) (FBrf0217163, reviewed in FBrf0233758). Upon expression of deGradFP, target proteins tagged with GFP (or any the other GFP derivatives bound by VHH(GFP4)) are specifically polyubiquitinated and rapidly degraded by the proteasome (adapted from FBrf0233758).

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Transgenic Constructs
Encodes tool (15)
Transgenic construct(s)
Component allele
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Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NSlmb-vhhGFP4
deGradFP
Name Synonyms
degrade Green Fluorescent Protein
Secondary FlyBase IDs
    References (17)