The GMR15A01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR71G01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R15A01 drives expression in 8-10 fru positive adult fruitless pMP-e (male) neurons 0-4 fru negative neurons in the lateral horn of both male and females when combined with ScerGAL4DBD.R71G01 or ScerGAL4DBD.R22G11.
In a split-GAL4 system, ScerGAL4DBD.R71G01 drives expression in 8-10 fru positive adult fruitless pMP-e (male) neurons 0-4 fru negative neurons in the lateral horn of both male and females when combined with HsapRELAAD.R15A01.