FlyBase Gene Report: Dmel\4Csp2

General Information

Symbol

Dmel\4Csp2

Species

D. melanogaster

Name

4-Cysteine soluble protein 2

Annotation Symbol

CG11390

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011695

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Protein component of the posterior mating plug. (UniProt, Q9W1C9)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

PebIII, EbpIII, PEBmeIII, Ejaculatory bulb protein III, DmelCSP2

Key Links

Genomic Location

Cytogenetic map

60B4-60B4

Sequence location

2R:24,026,172..24,027,518 [+]

Recombination map

2-106

RefSeq locus

NT_033778 REGION:24026172..24027518

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in metamorphosis

inferred from expression pattern

(Sabatier et al., 2003)

involved_in response to virus

inferred from expression pattern

(Sabatier et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mating plug formation

inferred from sequence or structural similarity with UniProtKB:Q9U6L5

(Gene Ontology Curators, 2002-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Sabatier et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from sequence model

(Vieira and Rozas, 2011)

non-traceable author statement

(Dyanov and Dzitoeva, 1995)

Gene Group (FlyBase)

4-CYSTEINE SOLUBLE PROTEINS

Protein Family (UniProt)

Belongs to the insect A10/OS-D protein family. (Q9W1C9)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

4-CYSTEINE SOLUBLE PROTEINS -: 4-cysteine soluble proteins are small proteins found in arthropods that mediate olfactory recognition at the periphery of sensory receptors as well as having non-chemosensory functions. They posses four neighboring cysteine residues that are highly conserved. (Adapted from PMID:41752605.)

Protein Function (UniProtKB)

Protein component of the posterior mating plug.

(UniProt, Q9W1C9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\4Csp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W1C9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

4Csp2-RA

FBtr0072164

NM_080227

586

126

4Csp2-RB

FBtr0343340

NM_001299879

768

126

4Csp2-RC

FBtr0343341

NM_001299880

836

126

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

4Csp2-PA

FBpp0072073

14.5

126

8.29

FBpp0309998

14.5

126

8.29

FBpp0309999

14.5

126

8.29

Q9W1C9

NP_001286809

AHN56604

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

126 aa isoforms: 4Csp2-PA, 4Csp2-PB, 4Csp2-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\4Csp2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.63

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

larval foregut atrium

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Sabatier et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\4Csp2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\4Csp2

View exonic expression by tissue for Dmel\4Csp2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011695

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49821/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011695

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011695

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011695

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11390

Flygut - An atlas of the Drosophila adult midgut

FBgn0011695

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of 4Csp2

Transgenic constructs containing regulatory region of 4Csp2

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2R)Exel6080

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2R)HSPC300^Δ31.1

(Qurashi et al., 2007)

Df(2R)HSPC300^Δ52.1

(Qurashi et al., 2007)

Df(2R)HSPC300^Δ96.1

(Qurashi et al., 2007)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

4Csp2^GD1192

viable, with Scer\GAL4^Act5C.PI

4Csp2^UAS.cSa

viable, with Scer\GAL4^da.G32

4Csp2^UAS.cSa

viable, with Scer\GAL4^Mef2.PR

4Csp2^GD1192

viable, with Scer\GAL4^pnr-MD237

4Csp2^GD1192

viable, with Scer\GAL4^yolk

4Csp2^UAS.cSa

Other Phenotypes

Allele

increased efficacy of long-term memory | adult stage, with Scer\GAL4^DBD.Mef2.247

4Csp2^HMC04221

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP008052

11 of 12

Yes

Agam\AgaP_AGAP008054

11 of 12

Yes

Agam\SAPP1

10 of 12

Yes

Agam\AgaP_AGAP008055

4 of 12

Yes

Agam\AgaP_AGAP008059

3 of 12

Agam\AgaP_AGAP008058

1 of 12

Yes

Agam\AgaP_AGAP008062

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:4Csp2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

4Csp3

6 of 13

4Csp4

6 of 13

4Csp1

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

4Csp2 - Antdh

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Nplp2

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Obp19d

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Obp44a

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Obp56d

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Obp99c

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - Syx6

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - apolpp

experimental knowledge based

(Guruharsha et al., 2011)

4Csp2 - frm

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72483

DroID - A comprehensive database of gene and protein interactions.

FBgn0011695

MIST (protein-protein) - An integrated Molecular Interaction Database

49821

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60B4-60B4

Sequence location

2R:24,026,172..24,027,518 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60B4-60B4

Limits computationally determined from genome sequence between P{lacW}Phm^k07623&P{lacW}tsr^k05633 and P{EP}EP503

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-109.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

55933

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04221}attP40

VDRC

v100157

P{KK105086}VIE-260B

v5622

w¹¹¹⁸; P{GD1192}v5622

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (258)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH74005

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011695

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011695

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Expression is induced in the haemolymph of DCV virus infected flies.

(Sabatier et al., 2003)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per⁰¹, tim⁰¹ and Clk^Jrk background.

(Claridge-Chang et al., 2001)

Relationship to Other Genes

Source for database merge of

Source for merge of: PebIII CG11390

(Bayraktaroglu, 2000.4.12)

Source for merge of: PebIII BcDNA:RH74005

(FlyBase, 1996-)

Additional comments

The Phk-3 and PebIII genes share at least 50% identity.

(Langille and Clark, 2007)

Source for merge of PebIII BcDNA:RH74005 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: EbpIII PebIII

(FlyBase, 2009-)

Source for identity of: Csp2 EbpIII

(Zhou et al., 2006)

Source for identity of: 4Csp2 Csp2

(Liu et al., 2026)

Nomenclature comments

Changed from 'PebIII' to 'EbpIII' to be consistent with change of 'Peb' to 'Ebp'.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

4Csp2

BcDNA:RH74005

CG11390

(Widmer et al., 2018, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Swanson et al., 2004, Robertson, 2000.4.13)

CSP2

(Pang et al., 2026)

Csp2

DmelCSP2

(Vieira and Rozas, 2011, Zhou et al., 2006)

EbpIII

(Tu et al., 2023, Xu et al., 2022, Ghosh et al., 2020, Hung et al., 2020, Widmer et al., 2018)

Ebsp3

(Liu et al., 2026)

PEB

(Lopez et al., 2001)

PEBmeIII

(Dyanov and Dzitoeva, 1995, Dyanov, 1994.4.1)

PEBmelIII

(Zhou et al., 2006)

PebIII

(Liu et al., 2026, Picimbon, 2026, McLaughlin et al., 2021, Picimbon, 2020, Zheng et al., 2011, Anholt and Williams, 2010, Langille and Clark, 2007, Qurashi et al., 2007, Vieira et al., 2007)

Phk-2

(Zhou et al., 2006, Sabatier et al., 2003)

phk-2

(Sabatier et al., 2003)

Name Synonyms

4-Cysteine soluble protein 2

Chemosensory protein 2

Ejaculatory bulb protein III

(Cammarato et al., 2011, Kim and Marqués, 2010)

Pherokine 2

(Sabatier et al., 2003)

ejaculatory bulb III

(Yadav et al., 2017)

Secondary FlyBase IDs

FBgn0034960
FBgn0062902

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49821

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W1C9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W1C9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

D5A7M1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W1C9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011695

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49821/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011695

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011695

FlyMine - An integrated database for Drosophila genomics

FBgn0011695

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11390

MARRVEL_MODEL - MARRVEL (model organism gene)

49821

Linkouts

BioGRID - A database of protein and genetic interactions.

72483

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011695

DroID - A comprehensive database of gene and protein interactions.

FBgn0011695

DRSC - Results frm RNAi screens

FBgn0011695

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011695

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011695

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11390

Flygut - An atlas of the Drosophila adult midgut

FBgn0011695

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

49821

References (57)

The following external sites may use different assemblies or annotations than FlyBase.

-0.63

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