FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Arpc5
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General Information
Symbol
Dmel\Arpc5
Species
D. melanogaster
Name
Actin-related protein 2/3 complex, subunit 5
Annotation Symbol
CG9881
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031437
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Actin-related protein 2/3 complex, subunit 5 (Arpc5) encodes one of seven components of the highly conserved Arp2/3 complex. The Arp2/3 complex is required for many cellular and developmental processes requiring F-actin polymerization, including germline ring canal growth, embryonic cellularization, myoblast fusion, and endocytosis. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

p16-ARC, p16

Key Links
Genomic Location
Cytogenetic map
23A1-23A1
Sequence location
Recombination map
2-7
RefSeq locus
NT_033779 REGION:2580292..2581423
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables protein-macromolecule adaptor activity
inferred from biological aspect of ancestor with PANTHER:PTN000291131
(GO Reference Genome Project, 2011-)
enables structural constituent of cytoskeleton
inferred from sequence or structural similarity with UniProtKB:O15511
(Hudson and Cooley, 2002)
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of actin filament polymerization
inferred from mutant phenotype
(Poukkula et al., 2014)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in Arp2/3 complex-mediated actin nucleation
inferred from biological aspect of ancestor with PANTHER:PTN000291131
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR006789, InterPro:IPR036743
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with UniProtKB:O15511
(Hudson and Cooley, 2002)
involved_in cell migration
inferred from biological aspect of ancestor with PANTHER:PTN000974138
(GO Reference Genome Project, 2011-)
involved_in regulation of actin filament polymerization
inferred from electronic annotation with InterPro:IPR036743
(InterPro Project Members, 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in actin cytoskeleton
inferred from electronic annotation with InterPro:IPR006789, InterPro:IPR036743
(InterPro Project Members, 2004-)
part_of Arp2/3 protein complex
inferred from electronic annotation with InterPro:IPR006789, InterPro:IPR036743
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with UniProtKB:O15511
(Hudson and Cooley, 2002)
inferred from biological aspect of ancestor with PANTHER:PTN000291131
(GO Reference Genome Project, 2011-)
is_active_in cortical cytoskeleton
inferred from biological aspect of ancestor with PANTHER:PTN000291131
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Actin-related protein 2/3 complex, subunit 5 (Arpc5) encodes one of seven components of the highly conserved Arp2/3 complex. The Arp2/3 complex is required for many cellular and developmental processes requiring F-actin polymerization, including germline ring canal growth, embryonic cellularization, myoblast fusion, and endocytosis. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
ACTIN-RELATED PROTEIN 2/3 COMPLEX ARPC3A VARIANT -
The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
ACTIN-RELATED PROTEIN 2/3 COMPLEX ARPC3B VARIANT -
The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Arpc5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VQD8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Arpc5-RA
FBtr0077694
NM_134849
785
151
Arpc5-RB
FBtr0335170
NM_001273016
1070
151
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Arpc5-PA
FBpp0077378
16.7
151
4.79
Q9VQD8
NP_608693
AAF51237
Arpc5-PB
FBpp0307163
16.7
151
4.79
Q9VQD8
NP_001259945
AGB92482
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

151 aa isoforms: Arpc5-PA, Arpc5-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Arpc5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.10

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Arpc5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Arpc5
Transgenic constructs containing regulatory region of Arpc5
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2L)Exel6008
(Ryder, 2004.4.26)
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
some die during pupal stage, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
leg disc | P-stage, with Scer\GAL4Dll-em212
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\ARPC5
14 of 14
Yes
Yes
1  
Hsap\ARPC5L
13 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Arpc5
14 of 14
Yes
Yes
Rnor\Arpc5l
13 of 14
No
Yes
Rnor\Arpc5l-ps1
10 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Arpc5l
13 of 14
Yes
Yes
Mmus\Arpc5
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\arpc5l
10 of 13
Yes
Yes
Xtro\arpc5
10 of 13
Yes
Yes
Xtro\mrpl42
1 of 13
No
No
Danio rerio (Zebrafish) (4)
Drer\arpc5a
14 of 14
Yes
Yes
Drer\arpc5b
14 of 14
Yes
Yes
Drer\arpc5la
13 of 14
No
Yes
Drer\arpc5lb
12 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\arx-7
14 of 14
Yes
Yes
Cele\C46H11.3
14 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP008895
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AT5G65274
11 of 13
Yes
Yes
Atha\CRK
11 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\ARC15
11 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\arc5
9 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Arpc5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Arpc5GD12571
    model of  candidiasis
    CEA
    (Glittenberg et al., 2022)
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    ARPC5; actin related protein 2/3 complex subunit 5
    model of  immunodeficiency 113
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ARPC5; actin related protein 2/3 complex subunit 5
    14 of 14
    604227
     
    ARPC5L; actin related protein 2/3 complex subunit 5 like
    13 of 14
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map
        2-7
        Cytogenetic map
        23A1-23A1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        23A1-23A1
        Limits computationally determined from genome sequence between P{PZ}dpp10638 and P{EP}EP832
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-7

        (FlyBase, 2016)

        2-5.4

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (12)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (32)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

            (Stroschein-Stevenson et al., 2006)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)
            Relationship to Other Genes
            Source for database merge of
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: p16-ARC CG9881

            (Goldstein and Gunawardena, 2000)

            Source for identity of: Arpc5 p16-ARC

            (FlyBase, 2009-)
            Nomenclature comments

            'p16-ARC' renamed to 'Arpc5' to rationalize the nomenclature of genes encoding subunits of the Actin-related protein 2/3 complex, following the nomenclature suggested in FBrf0144947 which is consistent with that used in mammals.

            (FlyBase, 2009-)
            Etymology
            Synonyms and Secondary IDs (9)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 41 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (57)